2nd International
Probiotics, Nutrition & Microbiome Conference

October 10-12, 2018 | Amsterdam, Netherlands

Program Schedule

  • Keynote Speaker

    Time:

    Title

    Title: Nutrition and Microbiome – Opportunities from a Public Health Perspective

    Stoffer Loman
    NutriClaim BV, The Netherlands
    Biography
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    Biography

    Stoffer Loman
    NutriClaim BV, The Netherlands

    Stoffer has acquired a BSc-degree in Tropical Agriculture at the Royal Dutch College for Tropical Agriculture (Deventer, the Netherlands, was trained a Nutritionist (MSc) at Wageningen University (1992) and has obtained his PhD in Medical Sciences/Immunology at the Academic Medical Centre of the University of Amsterdam/ (1998). Following a career in the food supplement industry as science communicator and health educator he founded NutriClaim in 2007 (www.nutriclaim.com), providing specialist services pertaining to the scientific substantiation of health claims made on food, and marketing authorization of Novel Foods in the EU. Currently, Stoffer is also Work Package Leader in the EU FP7-funded project “MyNewGut".



    Abstract
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    Abstract

    Stoffer Loman
    NutriClaim BV, The Netherlands

    Diet-microbiome interactions may be moderators of human metabolism. Does this mean that with the ingestion of a capsule with the mix of the relevant strains we can colonize our gut with beneficial organisms that prevent us from obesity, cancer, cardiovascular or metabolic diseases are obesity type 2 diabetes, ect….? This question will be considered and the hypothesis of the “dietary fiber gap” is proposed to (partly) explain the (causal?) relationship between the loss of diversity of the gut microbiome and the incidence of “modern diseases”, like obesity and type 2 diabetes. Implications for public health messages will be discussed.

    Keynote Speaker

    Time:

    Title

    Title:

    Abdolkarim Sheikh
    Dezful University of Medical Sciences, Iran
    Biography
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    Biography

    Abdolkarim Sheikh
    Dezful University of Medical Sciences, Iran

    Dr. Sheikhi started his B.Sc. in Shahid Chamran University in Biological Sciences and then his master in Medical Immunology in Tarbiat Modarres University. He did his Ph.D. at Immunology Institute, Kiel, Germany and Department of Immunology, Faculty of Medicine, Shiraz University of Medical Sciences (UMSc).Currently he is the Professor of Immunology, head of department of Immunology and Microbiology at Dezful University of Medical Sciences. He currently studying the effect of yogurt derived probiotics and some support nutrients on the immune response of ulcerative colitis (UC) patients.



    Abstract
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    Abstract

    Abdolkarim Sheikh
    Dezful University of Medical Sciences, Iran

    Ulcerative colitis (UC) a major form of inflammatory bowel disease (IBD), is a cause of significant morbidity worldwide and its incidence and prevalence appear to be increasing with time. Patients with UC frequently experience episodes of bloody diarrhea with or without mucous, abdominal pain, fever and weight loss.UC patients are faced with malnutrition due to numerous pathogenic factors including anorexia, malabsorption, altered metabolism, fluid and electrolyte loss and side effects of medications.That is why the nutrient supplements should be provided to avoid development of nutrient deficiencies.UC is assumed to be a result of a breakdown of tolerance to intestinal environmental antigens such as resident enteric bacteria and altered barrier properties of the mucous and epithelial layers. These alterations can allow luminal antigens to penetrate the intestinal mucosa and elicit an overproduction of pro-inflammatory cytokines and trafficking of effector leukocytes into the intestinal mucosa. This can ultimately lead to uncontrolled and exaggerated intestinal inflammation. This review will present and discuss the immune response of UC patients to different species of probiotics and nutrient supplements.

    Sessions:
    Industrial Production and Dairy Technology & Plants Probiotics, Nutrition and Synbiotics & Food Microbiology and Immunology & Nutritional Neuroscience & Eating Disorders & Probiotics in Healthcare, Bussiness and Regulatory Aspects

    Time:

    Title: Lactobacillus Acidophilus W37 Supports Intestinal Health and Immune

    Nicole de Wit
    Wageningen University and Research, Netherlands

    Biography
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    Biography

    Nicole de Wit
    Wageningen University and Research, Netherlands

    Dr.Nicole de Wit is a senior research scientist at Wageningen Food & Biobased Research with major expertise in gut physiology. At the department of Human Nutrition of Wageningen University, she previously explored the role of nutrition, and especially high fat diets, on intestinal physiology at the molecular level in various mouse models. In her current job at Wageningen Food & Biobased Research, she is involved in multiple projects that study the role of fibres and pre- and probiotics in gastrointestinal health using in vitro models as well as animal models (piglets) and human intervention trials. For instance, she studied the immunomodulatory effect of probiotic strains by exploring their potential to support vaccination efficacy. She isalso involved in public private partnership (PPP) projectsin which the role of nutrition is studied to improve quality of life of irritable bowel syndrome (IBS) patients and how personalized dietary advice on fibre intake can empower consumers to make the right choices for improved health or reduced health risk.



    Abstract
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    Abstract

    Nicole de Wit
    Wageningen University and Research, Netherlands

    Lactobacillus strainsare interesting probiotic strains which might have immune modulatory and other beneficial health effects, but each strain can be different in its activity. Here we tested three Lactobacillus strains, L. acidophilusW37,L. brevis W63 andL. casei W56, for their modulatory effects towards small intestinal epithelial cells in vitro and whether the most potent strain L. acidophilus W37 could have immune supportive effects in neonatal piglets. Caco-2 cells differentiated to small intestinal epithelial cells were exposed to L. acidophilus W37,L. brevis W63 and L. casei W56 (107 CFU/mL) for 6 hours. During exposure, trans epithelial electrical resistance (TEER) was measuredto determine the integrity of the confluent epithelialmonolayer. To determine the effect of theLactobacillus strains on gene expression and related canonical pathways, RNA of Caco-2 cells washarvestedafter 6 hours incubation, hybridized to Affymetrix Human Gene 1.1 ST arrays and Ingenuity Pathway Analysis (IPA) analysis was performed. Additionally, the potential beneficial effect of L. acidophilusW37 on intestinal integrity was studied using Caco-2 cells challenged with Salmonella Typhimurium (STM) DT12. In neonatal piglets, L. acidophilusW37 combined with long chain inulin type fructanFrutafit®TEX! was tested to enhance efficacy of vaccination against Salmonella Typhimurium. Treatments were given daily via oral drenches as of day 2 after birth until sacrifice. Piglets were weaned on day 24 and vaccinated with a single dose of Salmoporc STM®. To analyze the effect on protection againstSTM, animals were challenged with this pathogen before sacrifice. Blood was sampled prior and post vaccination to quantify antibody titers and characterisation of immune cells. In the Caco-2 cell model, L. acidophilus W37 enhanced TEER by 15% (p<0.01) after 6 hours, while the strains L. brevis W63 andL. casei W56 had almost no effect on TEER.Transcriptomic data also showed that L. acidophilusW37 induced much more pronounced effects then the other 2 Lactobacillus strains. Most genes modulated by L. acidophilus W37 were related to intestinal barrier function, inflammation and bacterial stimulation of the epithelial layer.Many genes involved in the regulation of tight junctions were upregulated (e.g. CLDN 3, -4, Rab13)which fits the change in TEER. L. acidophilus W37 seems to impact epithelial-immune interactions via NFkB signaling and the upregulation of markers of follicle-associated epithelium (FAE) indicates thatL. Acidophilus W37 might support epithelial cell differentiation towards tissue with enhanced intestinalimmune sampling capacity. Challenging Caco-2 cells with STM showed a drop in TEER of 24% (p<0.01) compared to medium control. This drop was only 3.9% (p<0.05) in cells pretreated for 17 hours with L. acidophilusW37 compared to medium control, illustrating the beneficial effect of this strain during STM infection.In piglets, the combination of L. acidophilus W37 andFrutafit® TEX! enhanced antibody titers against STM. This positive effect of L. acidophilus W37 on vaccination efficacy is indicative for its immunomodulatory potential. These results demonstrate that L. acidophilus W37can have beneficial effects on intestinal barrier function and aninteresting immune supportive activity. L. acidophilus W37 is therefore potentially useful to support intestinal health to e.g. STM burden in society and immune health for those groups that have a low immune fitness.

    Time:

    Title: Saccharumofficinarium (sugarcane) molassesenhancesTGF-β secretion and FoxP3 expression by Probiotic Yogurt Culture BifidobacteriumAnimalis Subsp. Lactis BB-12 stimulated PBMCs from Patients with Ulcerative Colitis

    Abdolkarim Sheikhi
    Professor, Dezful University of Medical Sciences, Iran

    Biography
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    Biography

    Abdolkarim Sheikhi
    Professor, Dezful University of Medical Sciences, Iran

    Dr. Sheikhi started his B.Sc. in Shahid Chamran University in Biological Sciences and then his master in Medical Immunology in Tarbiat Modarres University. He did his Ph.D. at Immunology Institute, Kiel, Germany and Department of Immunology, Faculty of Medicine, Shiraz University of Medical Sciences (UMSc).Currently he is the Professor of Immunology, head of department of Immunology and Microbiology at Dezful University of Medical Sciences. He currently studying the effect of yogurt derived probiotics and some support nutrients on the immune response of ulcerative colitis (UC) patients.



    Abstract
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    Abstract

    Abdolkarim Sheikhi
    Professor, Dezful University of Medical Sciences, Iran

    BACKGROUND: Ulcerative colitis (UC) is one of the inflammatory diseases of the gut with frequent bloody diarrhea leads to increased rates of anemia. Evidences indicate the immunomodulation disorders in the response to intestinal microbiota in UC. Although sugarcane molasses, rich in necessary minerals and vitamins could be a good support nutrient but its effect on immune system of UC patients is unknown. To determine how the immune system of UC patients responds to molasses this study planned. METHODS: Bifidobacteriumlactis(B. lactis) were cultivated on MRS broth. Peripheral blood mononuclear cells (PBMCs) of 12 UC patients separated by Ficoll-Hypaque centrifugation and co-cultured with UV killed bacteria and/or molasses in RPMI-1640 plus 10% FCS. The FoxP3 gene expression measured by real-time PCR. TGF-β and TNF-α were measured in supernatant of PBMCs by ELISA. RESULTS: Sugarcane molasses and B. lactis significantly augmented TGF-β compared to control (p<0.01 and p<0.001 respectively). The secretion levels of TGF-β by B. lactis plus molasses compared to B. lactis stimulated PBMCs was significantly higher (p<0.05) but the level of TNF-α after 2/4/12 h incubation of PBMCs with B. lactis plus molasses compared to B. lactis alone wasn't changed (p>0.2). The level of FoxP3 expression after treatment with molasses was increased significantly (p<0.05).Although FoxP3 expression after treatment with the molasses plus B. lactis was increased but it wasn't significantcompared to control. CONCLUSION: These data show that if sugarcane molasses added to B. lactis, not only would not increase the pro-inflammatory cytokine, TNF-α, but also augments the anti-inflammatory cytokine, TGF-β by PBMCs.Increasing the expression of the FoxP3could be due to stimulating the activity of the Treglymphocytes. Therefore, sugarcane molasses could be a safe support to compensate the lost nutrients in UC patients.

    Time:

    Title: Hypocholesterolemic Potential of Probiotic Bacteria Isolated from Human Breast Milk

    Bilqeesa Bhat
    University of Jammu, India

    Biography
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    Biography

    Bilqeesa Bhat
    University of Jammu, India



    Abstract
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    Abstract

    Bilqeesa Bhat
    University of Jammu, India

    Probiotics are live microorganism, which, when consumed in adequate amount, confer health benefits to the host (FAO/WHO, 2002). Health benefits conferred by probiotics are majorly includes defense against microbial infections, bioavailability of nutrients, development of systemic immunity, management of raised cholesterol, triglyceride levels, diabetes and related cardiovascular diseases (CVD). CVD has become a leading cause of global mortality. The probiotics affects the host's energy homeostasis by means of various mechanisms producing small biomolecules responsible for managing lipid profiles, and participating in the absorption, metabolism and excretion of bile salts, which directly regulates the serum cholesterol levels. Cholesterol is a vital substance of the human body; however, longstanding raised serum cholesterol levels may lead to development of atherosclerosis which poses a major risk for CVD. In study, investigation was carried out to hypocholesterolemic potential of human breast milk probiotic bacteria. Enterococcus faecalis K2 exhibited highest bile salt hydrolase activity (BSH; 31.82nmol/mL/min of glycine) and produced the highest amount of cholic acid from deconjugation of bile salts (28.24%) in vitro. The E. faecalis K2 showed significantly high cholesterol assimilation (63.29%), co-precipitation of cholesterol with deconjugated bile acids (41.64%), cholesterol removal via cell wall fraction (39.22%) and sequestered appreciable amount of cholesterol from mixed micelles. The probiotic candidates with characteristic probiotic properties and significant cholesterol lowering potential may be used for lowering the risk of CVD development.

    Time:

    Title: Probiotic Packaging: Turning Probiotic into Healthier Probiotic Dairy Foods through Nanencapsulation Cryoprotectents Biomaterials

    Reyed M Reyed
    City for Scientific Research and Applied Technology, Egypt

    Biography
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    Biography

    Reyed M Reyed
    City for Scientific Research and Applied Technology, Egypt

    Dr Reyed is a researcher at Scientific Research and Technology Application city. He has deep expertise in Probiotic Microbiology, water microbiology and water treatment technology application stemming from 25 years a Scientific Researcher over 10 years executive technical consultant for H20 bioprocess of corporate Safibiowater in Alexandria Egypt “Integrated Eco-solution”. He has contributed to European Desalination Society, International water association & Egyptian council society and Egyptian scientific syndicate.



    Abstract
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    Abstract

    Reyed M Reyed
    City for Scientific Research and Applied Technology, Egypt

    This is a principally significant moment in the development of probiotic and prebiotic research. There has been strong expansion in dairy food yield containing probiotics and/or prebiotics, and a number of them are maintained by clinical investigation showing health benefits. it is not astonishing that a lot of human microbiome isolates, many of which are obligatively anaerobic cultivate extraordinarily weakly outside their natural habitat and can only be studied using culture independent approaches. Therefore, the large-scale cultivation and subsequent storage of probiotic bacteria in high numbers often presents a major a problem that delays a process to the realization of their commercial potential. It is important that probiotic products meet appropriate international standards, and have properly concurrence and distinguished organisms, in their therapeutic activity throughout shelf-life -stable formulations that have been shown in well-designed clinical studies to confer defined health benefits on the consume. This article sets the bases for the creation of innovative technological developments in the production of Probiotic within packaging in which, packing technology designed to give response to a number of issues related to the feasibility, stability and bioactivity of probiotic for the food industry. These technologies aim to integrate the probiotics within new packaging and coating material concepts including novel integration technologies, hyper microencapsulation – entrapment and enzyme encapsulation and/or immobilization, and cryoprotectents agent to improve survivability during spary drying can greatly benefit in the pharmaceutical and biomedical sectors and from the unique properties of synthetic and biomass derived bioencapsualtion polymers.

    Time:

    Title: Prevalence of Bovine Babesiosis and Its Effect on Pcv in Jimma Town and its Surrounding, South Western Ethiopia

    Feyera Gemeda Dima
    Jimma University, Ethiopia

    Biography
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    Biography

    Feyera Gemeda Dima
    Jimma University, Ethiopia



    Abstract
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    Abstract

    Feyera Gemeda Dima
    Jimma University, Ethiopia

    Babesiosis is caused by intraerythrocytic protozoan parasites of the genus Babesia that infect a wide range of domestic and wild animals and occasionally man. A cross-sectional study was conducted in Jimma town and its surrounding veterinary clinics beginning from November 2015 to April 2016. The objectives of the study were to detect Bovine Babesiosis and to assess its effect on PCV. The Giemsa staining technique was used for the detection of parasites and hematocrit centrifugation to find out the effect of the parasite on the packed call volume of host animals in the study area. . A total of 384 blood samples were collected from randomly selected cattle and examined. Parasitological findings revealed that the overall prevalence of Bovine Babesiosis was 6.8% (26/384). Of the total infected cattle, 6.5% and 0.3% was B. bovis and B. begimina, respectively. The prevalence of the disease was assessed within major risk factors like age groups, sexes, body condition, tick infestation and PCV. But, statistically significant difference was observed (P<0.05) only in body condition, tick infestation and PCV. In conclusion, the results of this study have indicated that Bovine Babesiosis was light in the study area. Nevertheless, the finding of the current study recommends and encourages applying proper tick control and prevention so that to drop off the current challenges in the study area.

    Time:

    Title: Diversity and Functional Properties of Lactic Acid Bacteria Isolated from Philippine Burong Mustasa (Brassica juncea L.)

    Marilen Parungao Balolong
    University of the Philippines Manila, Philippines

    Biography
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    Biography

    Marilen Parungao Balolong
    University of the Philippines Manila, Philippines

    Dr. Marilen Parungao Balolong, is an Associate Professor at University of the Philippines Manila, Philippines. Her appreciation for Microbiology started during her undergraduate years when she took up BS Biology at UPLB (1995). She further honed her skills when she took MS Microbiology, minor in Biochemistry, at UPLB (2002) and her doctorate degree, DrPH Medical Microbiology, at UP Manila (2015). She completed her post Doctoral at Dankook University, Republic of Korea. Dr. Balolong is one of the recently listed top scientists of the University of the Philippines. Her well-cited works are published in international journals. Dr. Balolong is also a recipient of international awards in Thailand and Indonesia for her work on Probiotics and Functional Foods to Understand and Manage Obesity and Blood Glucose Levels. She is currently professionally affiliated with the following national and international societies: American Society for Microbiology, Korean Society for Microbiology and Biotechnology, Philippine Society for Lactic Acid Bacteria; Philippine Association for the Advancement of Science; Philippine Society for Microbiology, Inc.; Philippine Academy of Microbiology; Mycological Society of the Philippines; Philippine Network of Microbial Culture Collections.



    Abstract
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    Abstract

    Marilen Parungao Balolong
    University of the Philippines Manila, Philippines

    Recently, whole genome studies of certain lactic acid bacteria (LAB) described genes that encode for functional properties including production of enzymes and immunomodulation molecules and probiotic properties. However, most research reports focus on LAB from meat- or milk-based fermented foods. Fermented foods are an important part of human diet, containing lactic acid bacteria (LAB), which turn carbohydrates into lactic acid and other beneficial products. Since the Philippines is a home to numerous plant-based fermented foods, this study aims to discover the functional benefits that we derive from them. The bacterial diversity of Philippine burong mustasa was assessed by PCR-denaturing gradient gel electrophoresis (PCR-DGGE) of the 16S rRNA gene (16S rDNA). Resulting sequence analyses revealed that lactic acid bacteria (LAB) were dominant in the food samples. The LAB identified included Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus panis, Lactobacillus pontis and Weissella cibaria. Among these, L. fermentum, L. plantarum and W. cibaria are established probiotic bacteria, while L. panis and L. pontis are potential probiotic bacteria. Putative LAB isolates were also selectively isolated using GYC agar plates shown as colonies with surrounding halos and were confirmed using Gram stain and Catalase test. Functional properties including production of exopolysaccharide, cellulase and amylase as well as hydrophobicity and probiotic properties were conducted for all 334 LAB isolates using standard procedures. Probiotic properties include antimicrobial activity, production of bacteriocin-like inhibitory substances (BLIS) and acid and bile tolerance. Three LAB isolates were shown to have promising functional properties. Two of them were identified as Lactobacillus fermentum (P4-1 and P4-2) while the other was identified as Staphylococcus carnosus (P4-3) using 16S rDNA sequencing. Overall, this study showed the potential of plant-based fermented foods such as our Philippine burong mustasa, that harbors LAB with beneficial functional properties.

    Sessions:
    Microbiota and Central Nervous System Development & Probiotics and Allergic Diseases & Practical Applications of Probiotics for Gastrointestinal Disorders & Accelerating Research in Probiotics

    Time:

    Title: Mechanisms Underlying Anti-Diarrheal Effects of Probiotics in Models of Infectious and Inflammatory Bowel Diseases (IBD) Associated Diarrhea

    Pradeep K Dudeja
    University of Illinois at Chicago, USA

    Biography
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    Biography

    Pradeep K Dudeja
    University of Illinois at Chicago, USA

    Dr. Dudeja is a Professor of Physiology in the Department of Medicine at University of Illinois at Chicago and a Senior Research career Scientist at the Jesse Brown VA Medical Center. His group focuses on pathophysiology of diarrheal diseases as it pertains to infectious & IBD associated diarrhea and to develop better therapeutic interventions. His recent studies have focused on defining the mechanisms underlying potential antidiarrheal effects of probiotics. He has published about ~220 original articles and has been supported by multiple grants from NIH and the Department of Veterans affairs. He serves as an Editor for “intestinal Absorption” for Comprehensive Physiology journal and on many editorial boards including: Gastroenterology, Amer. J. of Physiol., Physiological reports and Cell. & Mol. Gastroenterology & Hepatology. He has also served on many peer review committees including National Iinstitute for Health, and Department of Veterans Affairs, USA. He has been honored by Mario Toppo Distinguished Scientist Award from the ASIOA (Assoc. of Scientists of Indian Origin in America) and is a winner of Takeda Distinguished Scientist Award of American Physiological Society for 2018. These awards honor life-time achievements of Dr. Dudeja in the area of gut-microbe interactions in pathophysiology of diarrheal diseases.



    Abstract
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    Abstract

    Pradeep K Dudeja
    University of Illinois at Chicago, USA

    Introduction: Many Lactobacillus species have been successfully used in clinical trials to treat diarrhea in children. However, mechanisms underlying anti-diarrheal effects of probiotics are not well understood. Recent studies from our group have shown that certain lactobacillus and bifidobacterial species upregulated electrolyteabsorption in the intestine.Since Intestinal epithelial apical membrane NHE3 (Na+/H+ exchanger 3) and DRA (Down Regulated in Adenoma, a key Cl-/HCO3- exchanger) play key roles in mediating intestinal electroneutral NaCl absorption, we sought to evaluate the efficacy of Lactobacillus acidophilus(LA) in counteracting NHE3 and DRA inhibition and ameliorating diarrhea in a model of C rodentium infection and DSS induced colitis. Methods: FVBN mice challenged with C. rodentium (1x109 CFU) with or without administration of live LA (3x109 CFU) were assessed for NHE3 and DRA mRNA and protein expression, mRNA levels of carbonic anhydrase, diarrheal phenotype (assessed by colonic weight/length ratio), myeloperoxidase (MPO) activity and proinflammatory cytokines. For colitis studies, dextran sulfate sodium (DSS in drinking water for 7 days) was used to induce colitis in C57BL/6J mice. Results:LA counteracted C. rodentium-induced inhibition of colonic DRA, NHE3 and carbonic anhydrase I and IV expression, attenuated diarrheal phenotype and MPO activity. Further, LA completely blocked C. rodentium induction of IL-1, IFN- and CXCL1 mRNA and C. rodentium-induced STAT3 phosphorylation. Oral gavage of live LA also showed evident alleviation of inflammatory state by reducing the weight loss, decreased colon length and colon weight in both the models. Also,the inflammation as well as the decrease in DRA mRNA and protein levels caused by DSS colitis was blocked by LA. Conclusions:Our data provide mechanistic insights into anti-diarrheal and anti-inflammatory effects of probiotics in models of infectious and chemical injury colitis.

    Time:

    Title: Effect of Probiotics and Prebiotics on Gut Integrity in Critically Ill Patients

    Daniyah Abdullah Alkhawtani
    Prince Sultan Military Medical City, Saudi Arabia

    Biography
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    Biography

    Daniyah Abdullah Alkhawtani
    Prince Sultan Military Medical City, Saudi Arabia

    Daniyah Abdullah Alkhawtani is a clinical dietitian at Prince Sultan Military Medical City, Riyadh, Saudi Arabia. She obtained Bachelor of clinical nutrition from King Saud University, College of Applied Medical Sciences, Riyadh in 2011.She began her Master of clinical nutrition in 2013. Furthermore, she obtained Diploma in Sport and Exercise Nutrition. She Participated as a speaker in the advance course for resident's level 3(2015), 5th Clinical and Community Nutrition Update in Riyadh, Saudi Arabia(2016), 8th International Conference of Clinical Nutrition in Dubai (2016), 6th International Conference and Exhibition on Probiotics, Functional and Baby foods. London, UK, October 2017 and in 1st Annual Scientific Meeting of Saudi Arabia Parentral and Enteral Nutrition (SAPEN). Madareem Crown Hotel. Riyadh, Saudi Arabia.



    Abstract
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    Abstract

    Daniyah Abdullah Alkhawtani
    Prince Sultan Military Medical City, Saudi Arabia

    Gut failure is common in ICU patients which characterized by lack of bowel sounds, regurgitation, vomiting, high gastric residual volumes (>500 mL/day), diarrhea, constipation, abdominal distension or GI bleeding.During critical illness, several factors might affect gut microflora that involve changes in stress hormones, gut ischemia, use of antibiotics and immunosuppression, gut micrbiota, lack of nutrientsand enteral feeding failure.Unfortunately, clinical evaluation of the gut function is difficult therefore gut dysfunction usually goes unrecognized related to poor clinical outcomes and high morbidity and mortality rate.Diet has the strongest effects on gut microbial colonization that could modify the profile of dominant species in human gut and offer different consequences of health. Recent data suggested to preserve or reestablish a healthy gut microbiota during and after critical diseases through targeted interventions such as probiotics, prebiotics, fecal microbial transplants(FMT), and synthetic ‘stool pills’. This review developed to evaluate the efficacy of probiotic/prebiotics in critical ill patients. Probiotics offers many benefits to the host including effective in the treatment or prevention of acute gastroenteritis, GI dysfunctions, antibiotic associated diarrhea(AAD), certain pediatric allergic disorders, necrotizing enterocolitis and inflammatory bowel disease (IBD).Briefly, probiotics use in the ICU remains widespread and no definitive recommendation for the routinely probiotics use in critical ill patients. Further studies are required to define the dose, types and safety of pro- and prebiotics in critically illness.

    Time:

    Title: Dysbiosis of Microbiome and Probiotic Treatment in a Genetic Model of Autism Spectrum Disorders

    Laure Tabouy
    Bar-Ilan University, France

    Biography
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    Biography

    Laure Tabouy
    Bar-Ilan University, France

    After sustained her PhD in 2012 in molecular and cellular neuroscience and structural biology within the functional and adaptive biology unit of Paris Diderot-Paris 7 University, Dr. Laure Tabouy taught at the Faculty of Sciences of the Franche-Comté University, then went for a post-doctorate in Israel, working on the role of the microbiome and the Gut-Brain axis in autism and the establishment of a probiotic treatment, in the Molecular and Behavioral Neuroscience laboratory of Dr. Evan Elliott, in the faculty of Medicine in the Galilee of the Bar-IlanUniversity. She continues to work on the microbiota in France at the Neuroscience Institute of Paris Saclay, in France, (NeuroPSI)



    Abstract
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    Abstract

    Laure Tabouy
    Bar-Ilan University, France

    Background: The gut microbiome may influence brain development and behavior, mainly through the modulation of physiological metabolism and the immune system. Recent studies have determined that the microbiome has direct effects on behavior, and may be dysregulated in neurodevelopmental conditions. Considering that neurodevelopmental conditions, such as autism, have a strong genetic etiology, it is necessary to understand if genes associated with neurodevelopmental disorders, such as Shank3, can influence the gut microbiome, and if probiotics can be a therapeutic tool. Methods / Results: Using 16S high-throughput sequencing, we have determined the gut microbiome community of the Shank3 KO mouse model, and itsrelative controls. In this study, we have identified dysregulation of several genera and species of bacteria in both the gut and colon of Shank3 KO mice, in addition to a sex-dependent dysregulation of the immune system. L. reuteri, a species with decreased relative abundance in the Shank3KO mice, positively correlated with the expression of GABA receptor subunits in the brain. Treatment of Shank3 KO mice with L. reuteri induced an attenuation of unsocial behavior and a decrease in repetitive behaviors, in males and just decrease in repetitive behaviors in females, without affecting anxiety. L. reuteri treatment also induced an increase in GABA receptor expression in multiple brain regions, and affected serum immune system markers. Perspectives: This study has confirmed that genetic differences associated with autism can induce changes in the microbiota profile. In addition, this study identifies bacterial species that are sensitive to an autism-related mutation, and further suggests a therapeutic potential for probiotic treatment.

    Time:

    Title: From Microbiota-Gut-Brain-Axis to Live Biotherapeutic Products as Treatment for Neurodevelopmental / Neurodegenerative Diseases

    Pierre Lestage
    NeribiOm, France

    Biography
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    Biography

    Pierre Lestage
    NeribiOm, France

    Pierre Lestage is the CEO of NeribiOm, France.



    Abstract
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    Abstract

    Pierre Lestage
    NeribiOm, France

    Despite our limited understanding of the complex biology underlying the Microbiota-Gut-Brain axis (MGBA) function and physiology, during the last decade, preclinical and clinical studies have demonstrated that gut microbiota could influence Central Nervous System (CNS) physiology and function. For example, in various psychiatric disorders, studies have shown that gut dysbiosis is observed and that probiotics (psychobiotics) and prebiotics could alleviate symptoms in mood disorders. Recently, gut dysbiosis has been shown in neurodevelopmental (Autism,...) and neurodegenerative diseases (Alzheimer's, Parkinson's, Multiple Sclerosis,...). Moreover, very early during the time course of certain neurological diseases (Autism and Parkinson's) gastrointestinal symptoms appear. It should be also pointed out that aggregation of α-syncluein in Parkinson's disease is firstly observed in gut neurons. Furthermore, Aging, a period of instability for gut microbiota, is the major risk factor of dementias. Taking into account the mechanistic pathways underlying the communication between the gut and the brain, and the physiopathology of neurodegenerative diseases, we postulate that modulating gut microbiota using Live Biotherapeutic Products (LBPs) could be an innovative, disruptive, safe and pleiotropic way to treat very effectively neurological diseases. In this context, we have built an original collection mainly constituted of strict anaerobic bacterial strains from human faeces. These bacterial strains have been selected regarding their in vitro capacities i) To release neurotransmitters/neuromodulators, Short Chain Fatty Acids,..., ii) To have anti-inflammatory and immunologic activities,..., iii) To act on permeability of biological barriers,... Moreover, the selected strains have been tested regarding antibiotics' resistance. Some examples of this strategy will be described. Results allowed to obtain new innovative LBPs which are into preclinical studies as potential novel treatments against Autism Spectrum disorders and Parkinson's disease.

  • Keynote Speaker

    Time:

    Title

    Title: ProbioSatys – Naturally modulating the appetite via the microbiome.

    Gregory Lambert
    TargEDys, France
    Biography
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    Biography

    Gregory Lambert
    TargEDys, France

    Pharmacist and Ph.D. in pharmaceutical technology, Gregory LAMBERT carrier is split between Biotech and Pharma where he occupied the positions of Chief Scientific Officer and Chief Executive Officer in companies such as Novagali Pharma, Septodont and currently TargEDys. Gregory drove the development of several products from the laboratory to market authorizations. In 2014, Gregory joined the scientific founders of TargEDys as CEO. Having completed its preclinical development and produced industrial batches, TargEDys now concentrates on human clinical trials and commercial launch activities.



    Abstract
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    Abstract

    Gregory Lambert
    TargEDys, France

    TargEDys is a French biotech company aiming to control metabolic disease by modulating the appetite through an intervention on the microbiome. TargEDys’innovative,satiety inducing technology (ProbioSatys),is based on a unique understanding of appetite regulation at the molecular level. Bacteria can send signals of satiety to the brain from the gut by molecularly mimickingsatiety hormones, thus activating natural satiety pathways. This gut-brain axis is the bidirectional communication between the central and enteric nervous systems, linking the emotional and cognitive centres of the brain with peripheral intestinal functions. Gut bacteria are very important in influencing these interactions. The basis of TargEDys’ ProbioSatys technology is a commensal, enterobacteria probiotic strain, Hafnia alvei,that produces the ClpB protein. ClpB is a mimetic of the satiety hormone (α-MSH) that regulates food behaviour at both peripheral and central levels. When released, ClpB directly stimulates the intestinal enteroendocrine L-cells to produce satiety hormones. ClpB also enters the bloodstream to act on satiety regulation in the central nervous system by mimicking α-MSH. The resulting effect is the feeling of satiety or fullness after a meal. The mechanism of Hafnia has been proven preclinicallyin vitro and in vivo and is currently being tested in humans. This probiotic will be launched as a food supplement in April 2019. The preclinical results indicate that after 3 months of treatment customers can expect to safely lose 3-5% of their body weight, see a 5-10% reduction in food intake, improved body composition and activation of lipolysis.

    Keynote Speaker

    Time:

    Title

    Title: Personalised Nutrition for Healthy Consumers and the Market Opportunities

    Nard Clabbers
    TNO, Netherlands
    Biography
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    Biography

    Nard Clabbers
    TNO, Netherlands



    Abstract
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    Abstract

    Nard Clabbers
    TNO, Netherlands

    Nutrition is a major determinant for health and health is extremely important for all people. This means that there is value in making it possible that consumers can eat healthily with ease. The consumer of the future will have increased opportunities to choose according to their personal needs and demands by using self-generated data and technology via smart business models. Buzzwords to describe this development are the empowered consumer and information literacy. Healthy products and especially novel services that help consumers make the best choice will be a big part of this value chain. This is true both for healthy consumers and people that are under medical treatment because sometimes food is the best medicine. The evolving science of microbe-host interaction can be used to add value to consumers via novel products and services.

    Sessions:
    Probiotics in Productive Animals & Global and Public Health Nutrition & Obesity and Weight Management & Human microbiome, Diet and Health & Fecal Microbiota & Probiotics in Cancer Prevention and Treatment & Probiotics in Cosmetics & Skincare & Nutrigenomics & Personalised Nutrition

    Time:

    Title: Modulation of Gut Microbiome - From Phenomenology to Dietary Recommendations to Tackle Obesity

    Stoffer Loman
    NutriClaim BV, The Netherlands

    Biography
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    Biography

    Stoffer Loman
    NutriClaim BV, The Netherlands

    Stoffer has acquired a BSc-degree in Tropical Agriculture at the Royal Dutch College for Tropical Agriculture (Deventer, the Netherlands, was trained a Nutritionist (MSc) at Wageningen University (1992) and has obtained his PhD in Medical Sciences/Immunology at the Academic Medical Centre of the University of Amsterdam/ (1998). Following a career in the food supplement industry as science communicator and health educator he founded NutriClaim in 2007 (www.nutriclaim.com), providing specialist services pertaining to the scientific substantiation of health claims made on food, and marketing authorization of Novel Foods in the EU. Currently, Stoffer is also Work Package Leader in the EU FP7-funded project “MyNewGut.



    Abstract
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    Abstract

    Stoffer Loman
    NutriClaim BV, The Netherlands

    The potential role of intestinal microbiota in the etiology of various human diseases has attracted massive attention in the last decade. Experiments in animal models have produced evidence for a causal role of intestinal microbiota in the etiology of obesity and insulin resistance. However, with a few exceptions, such causal relation is lacking for humans and most publications merely report associations between intestinal microbial composition and metabolic disorders such as obesity and type 2 diabetes. Dietary recommendations are generally based on epidemiological evidence of an association between a food, food component or diet with the prevalence of a disease. However for microbiome-targeted dietary recommendations, little or no epidemiological evidence is available. In fact, world-wide no microbiome-related dietary recommendations have been proposed. Nevertheless, diet-induced changes in the microbiota have been shown to be associated with insulin resistance and development of diabetes. However, it is as yet an enigma as to which extend the altered microbiota is causally related to insulin resistance and diabetes or whether these changes in the microbiota are secondary to the development of insulin resistance and diabetes, the latter two induced by non-microbiota dependent pathophysiological mechanisms. Thus, the reciprocal relationship between the gut bacteria and these metabolic disorders remains a matter of debate. In addition, and in particular with respect to fiber, the health benefits of its consumption may be conferred even without the apparent involvement of the microbiome. In particular, e.g. studies into the role of fiber in its capacity to modify the microbiota to confer a health benefit have until now solely relied on measuring the changes in the microbiota at the start and completion of the study. Therefore, changes in the microbiota by itself are not sensitive enough to provide for a causal explanation of the observed metabolic effects. Unless multiple time points are included in the study design, kinetic of changes in the microbiota, combined with simultaneously revealing the kinetics of gene, protein and metabolome expression, applying multiple -omics techniques, microbiome-targeted dietary recommendations will remain elusive.

    Time:

    Title: Tackling Malnutrition, Obesity and Diabetes in Asia through Public Private Partnerships

    B. Kistner
    Defugo Bioceuticals, Germany

    Biography
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    Biography

    B. Kistner
    Defugo Bioceuticals, Germany



    Abstract
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    Abstract

    B. Kistner
    Defugo Bioceuticals, Germany

    Introduction: It is estimated that the world´s population will cap in the year 2100 at 11 bio people of which 90 % will live in Asia and Africa. The pressure on air, space, water and food supply will be immense. For a company in the food and beverage industry it is evident that business growth will come from Asia and Africa. Today both regions face nutrition related health issues, both a problem for society but also an opportunity for the F&B industry to develop innovate and healthy food solutions and join the public sector in Public Private Partnerships to develop product innovation, new business models and form alliances. Background: Aiming to contribute to Sustainable Development Goals SDG 2 & 3 – improving nutrition and SDG 17 partnerships. Today approximately 2/3 of humanity is affected by malnutrition – the fact that people have no access to food variety. Most devastating effects are during the first 1000 days of a human’s life – from conception to the 2nd birthday. If a human being has no access to a balanced diet during this time the person will either suffer from under-nutrition, stunting and wasting, have impaired brain capacity or will be overweight. In most Asian countries 30 % of children are stunted – too short for their age. Obesity is lowest in Asia, between 10 and 15 % of the population, however, the increase in Asia is the highest with almost 30 % in the last 4 years as a by-product of economic development. Obesity often results in other health problems like diabetes II, colon cancer or heart disease. The cost of obesity to the healthcare systems in Asia are up to 20 % of the total healthcare expenditure. Another health problem in Asia is osteoporosis – it is estimated that one in three women in Asia over 50 will suffer from it – this is mainly due to low milk consumption and no exposure to sunlight. Mission: The public debate tends to be dominated by single solution discussions, which often polarize groups, fan outrage and make constructive collaborations difficult. What is needed is a system change to facilitate healthy food choices for a population in general. We must engage in multi-sectoral dialogues between industry, academia, government and non-governmental organizations. Further each involved party needs to be ready to change, this is essential for development. Multiple, complementary interventions are necessary to show results. Studies are required on how to implement such interventions in different geo-political, social, physical, economic and cultural context. Interventions not as a linear process but rather holistic as a more circular process involving continuous learning, adaption and mainstream transparency. Public Private Partnership Process: • Start with data • Identify shared interest • Design for impact • Mainstream transparency • Measure outcomes

    Time:

    Title: Healing Autoimmune Diseases with Plant—Based Skincare

    Paul Joseph Griffith
    Vegan Skin, USA

    Biography
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    Biography

    Paul Joseph Griffith
    Vegan Skin, USA

    Paul Joseph Griffith has completed his Masters at Tufts University, serves as the C.E.O. of Vegan Skin by Paul Joseph, is a part time professional dancer, a former public high school teacher and lives a busy vegan lifestyle in Washington D.C., U.S.A.



    Abstract
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    Abstract

    Paul Joseph Griffith
    Vegan Skin, USA

    Vegan Skin by Paul Joseph is an all natural, plant-based, e-commerce, skincare, company that specializes in healing and hydrating the skin and scalp. Founder and C.E.O. Paul Joseph Griffith was diagnosed with a rare, autoimmune, skin, disease known as Hidradenitis Suppurativa when he was 23 years old. There is no known clinical cure or effective treatment for the disease. It is also degenerative and believed to affect approximately 1-2% of the American population and possibly globally. Throughout his formative years, the disease progressed and eventually spread across half of his body. He was covered in boils from the waist to his ankles and contemplated ending his own life. He decided in that moment to figure out a solution for himself driven by the research question, “How did our ancestors take care of themselves before modern medicine and technology?” Armed with this driving question, for four years he researched the healing properties of plants, focusing on their microbiology, and created three, signature, products for the skin and scalp. The products assisted in his healing and as a result became popular amongst friends and family members. Before long, the products became popular amongst customers across America with reports of assisted healing with autoimmune diseases such as eczema, psoriasis, dermatitis, folliculitis, cystic acne, chronically dry skin, rashes, and more. Thus, the focus of the oral presentation will be the intersection of the following: 1. The power of self—healing 2. The market for plant—based, natural, and wholistic solutions for skin and health 3. Plant microbiome and autoimmune disease treatment

    Time:

    Title: Vitreoscilla filiformis, A Spa Spring Water Bacterial Strain Used Since Centuries As An Original Translation of Probiotics’ Benefits to Skincare

    Lionel Breton
    L’Oreal Research, France

    Biography
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    Biography

    Lionel Breton
    L’Oreal Research, France

    Dr Lionel Breton got a PhD in 1982 in general Endocrinology in the lab of Pr R.Michel and Pr J.Roche at College de France in Paris. Then, he got a State Doctorate in general Pharmacology in Poitiers Hospital in 1985. After a 1st position in Servier Laboratory as Project leader in neuro-pharmacology, Lionel joined L’Oreal corporate 25 years ago. He is also visiting Professor/affiliate member in the prestigious University McGill in Montreal. He is an expert on neurobiology, skin neuro-immunology, skin physiology, skin and gut microbiology and translational research. He is currently Scientific Director in L’Oreal Research. Lionel is co-inventor of more than 1000 national patents and applications (espacenet results/world database) and 3 blockbusters of l’Oreal corporate. Although Industrial, he has published more than 50 papers in in peer-reviewed journals and is frequently invited to give plenary conferences or keynotes.



    Abstract
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    Abstract

    Lionel Breton
    L’Oreal Research, France

    Atopic xerosis and dandruff are both sub-inflammatory skin disorders driven by immune dysfunctions and are characterized from early stages by itch and delay in healing. As recently described, these two disorders are associated with specific dysbiosis of the skin microbiome with altered microbial diversity characterized by excess of Staphylococcus spp. or Malassezia spp., respectively. In skin aging as a subclinical condition, a dysbiosis was also recently described with an overabundance of oral bacterial strains usually not present on the skin together with a decrease of a commensal strain (Cutibacterium acnes). These dysbiosis and associated clinical signs may be controlled by Toll Like Receptor (TLR) specific ligands. The notion of dermatological thermal water-based treatments is dating back to Ancient Greece and the Roman Empire. In 1950, French scientists created a new skincare product using a mineral water from the thermal springs of Molitg-les-Bains in the Pyrenees mountains. They explained the skin rejuvenating and protecting activities of this product by the presence of a living specific non-pathogenic filamentous bacterial strain unique to these springs: Vitreoscilla filiformis. Clinical studies published in many journals of dermatology validated that this harmless bacteria could improve skin health and confer benefits similar to probiotics for gut health. The action mechanism of Vitresocilla filiformis strain has recently been understood: it contains TLR ligands which regulate the skin barrier function and skin innate defenses through microbiome balance. As also known from probiotics, this TLR signaling induces tolerogenic interleukin-10 which may explain the clinical effects of Vitreoscilla filiformis..

    Time:

    Title: From digestive health to microbiome modulation: learnings from lactulose / galactofructose

    Pascal Ronfard
    President, Groupe SOLACTIS, France

    Biography
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    Biography

    Pascal Ronfard
    President, Groupe SOLACTIS, France

    Since 2013, Pascal Ronford has positioned Groupe SOLACTIS as a leader of food and feed ingredients, active on the microbiota. This positioning has been rewarded by highly sought regulatory recognitions: 2 European (EFSA) and 1 South Korean (MFDS) official health claims. In 2015, Groupe SOLACTIS has located its R&D center in the renowned Science Campus INRA, near Paris. Mr. Ronfard is President of the Professional Group “HEC- Agro”, and member of the board of Club PAI. Mr. Ronfard is a graduate of the HEC Business School and holds a Master degree in Corporate Law, from Paris II University.



    Abstract
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    Abstract

    Pascal Ronfard
    President, Groupe SOLACTIS, France

    Firstly identified as a “bifidogenic factor”, then developed as an ingredient active on the intestinal transit regulation, lactulose or galactofructose is a diversified substance with a unique history. In our research at Groupe Solactis, we have firstly focused on intestinal transit effects, as a food ingredient, and defined limits vs . pharmaceutical effects. A regular intestinal transit time may be difficult to define, and galactofructose (or lactulose) is an efficient bowel regulatory ingredient for food applications, which enables to deepen the knowledge on transit time regulation.. In a second step, we have gathered every scientific evidences of the impact of lactulose galactofructose on the microbiota. This molecule cannot be used by small bowel enzymes and reaches the colon intact. There, “good bacteria” as Lactobacillus and Bifidobacterium can utilize galactofructose lactulose and grow selectively. We have also studied the data on in-vitro interaction with probiotic bacteria. Comparing the results open a key line of research: chicken or eggs, what comes first between intestinal transit regulation, and control of microbiota bacterial population and balance? This unique combination opens very powerful new food ingredients, active both on intestinal transit regulation and microbiota modulation. Galactofructose is the pioneer, and other molecules are joining. Finally, this approach adapted to animal feed industries paves the way for some reliable key innovations in this second industry. Science is making huge steps on microbiota - making it accessible to food and feed industries is the target.

    Time:

    Title: Efficacy of Lactobacillus Rahmnosus CRD11 against DMH Induced Colon Cancer in Rat Model

    Chand Ram Grover
    ICAR-National Dairy Research Institute, India

    Biography
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    Biography

    Chand Ram Grover
    ICAR-National Dairy Research Institute, India

    Prof. (Dr.) Chand Ram Grover obtained his Doctorate & Masters’ degrees in Dairying (Microbiology) from ICAR-National Dairy Research Institute, Karnal-132001, (Haryana), INDIA. He joined Agricultural Research Service in 1997 as scientist after completion of doctorate program. Presently he is holding the post of principal scientist (Dairy Microbiology. His research priority areas include development of functional foods w.r.t. preventive health care microbiology, especially prevention of colorectal cancer and non alcoholic fatty liver diseases, food safety (bioremediation of microbial toxins and toxic metals), probiotic direct vat starter (DVS) technology, bio-preservation of dairy foods by application of bio-molecules and mitigation of antimicrobial resistance in food borne pathogens. He has published >100 research/ review/ technical articles/ conference proceedings in referred international and national journals. He is recipient of a number of best research awards by various scientific societies such as Indian Dairy Association; Association of Food Scientists and Technologist (India), Friendship Forum of India, United Writers Association of India. He is the “Fellow of United Writers Association of India”. He has served Association of Food Scientist &Technologist (India) in various capacities (Vice President & Secretary) to create awareness among general public and food professionals about latest developments in food science. He is on expert panel of many agricultural/ academic universities such MPKV, Rahuri (MS); West Bengal University of Animal and Fisheries Sciences, Kolkata (WB); MPUAT, Udaipur (Raj) ; Chhattisgarh Kamdhenu Vishwavidyalaya, Durg (CG); Assam Agricultural University, Assam; Kurukshetra University, Kuruksetra (Haryana), Karnataka Milk Federation (Karnataka); Indian Council of Agricultural Research, New Delhi; GADVASU, Ludhiana (Pb) etc. Currently he is working on development of microbial formulations for bioremediation of toxic compounds and development of function foods.



    Abstract
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    Abstract

    Chand Ram Grover
    ICAR-National Dairy Research Institute, India

    Colon cancer accounts over 1 million people & about 0.5 million death worldwide annually. Putrefactive bacteria play a vital role in diet related carcinogenesis by conversion of pro-carcinogen into carcinogen. Dietary modification may offer opportunity to prevent this menace. Present investigation was aimed to evaluate efficacy of lactobacilli of Indian origin in format of blueberry fortified probiotic dahi against 1, 2,-dimethyl hydrazine (DMH) induced colon carcinoma in rats. In vitro 29 lactobacilli strains were evaluated for anti-carcinogenic potentials by Ames test and antioxidative assay. Among these, 6 selected strains were validated for probiotic and techno-functional properties. Lactobacillus rhamnosus CRD11 and L. plantarum CRD2 exhibited anti-carcinogenic and techno-functional potential. Finally, L. rhamnosus CRD11 was selected for preparation of blueberry fortified probiotic dahi (BPD) based upon its compatibility with it. Response surface methodology (RSM) was applied by using rotatory design package 9.0.1 for optimization of conditions for preparation of BPD. The RSM recommended best combination of 8% blueberry pulp, 1.5 % inoculum level and 9 h incubation time for development of BPD. Developed formulation was analyzed for its sensory & overall acceptability on 100 point scale revealed overall acceptability score of 88.79 with shelf life of 21 days at 4±1⁰C storage temperature. Probiotic dahi (PD) and blueberry fortified probiotic dahi (BPD) along with control dahi (NCDC 167) efficacy was validated against DMH induced colon carcinogenesis in male wistar rats w.r.t. histological, biochemical, microbiological and molecular markers. Probiotic dahi alone and in combination with blueberry effectively prevented colon cancer as tumor incidence, multiplicity and volume were lower in both BPD and PD fed group. Both, PD and BPD fed group exhibited decrease in progression of preneoplastic biomarker such as aberrant crypt foci (ACF) and mucin depleted foci (MDF) and BPD dahi documented synergistic efficacy as compared to probiotic dahi alone. The PCNA labeling index, a marker for progression of colon carcinoma in GI tract increased progressively in rats challenged with DMH. Both PD & BPD exhibited synergistic effect by preventing rise in PCNA & p53 labeling index. The BPD fed group showed higher antioxidant (catalase & superoxide dismutase) and detoxification (glutathione-s-transferase) activity as compared to DMH group. The fecal β- glucuronidase activity was higher in DMH control group in comparison to BPD and PD fed groups. Gene expression study of bcl2, k-ras, c-myc, and cox-2 gene in rat colon tissues expressed their higher levels in DMH control group as compared to BPD &PD groups as latter two groups showed minimum genotoxic stress. Similarly, histological analysis revealed higher incidences of carcinoma tumors in DMH control group while no carcinoma was observed in PD and BPD fed groups. Inhibition of colon cancer was more in BPD fed group as compared to only PD fed group. Blueberry has ability to stimulate growth of used probiotic strain in preparation of BPD. Hence, it can be concluded that there might be synergistic interactions between blueberry and beneficial microbes in the colon as evidenced by enhanced protective effect against colon carcinogenesis. Present investigation has culminated into development of anti-carcinogenic blueberry fortified probiotic dahi. Hence, L. rhammnosus CRD11 and blueberry offers exciting opportunities in food and health industry for the development of health foods to improve health of population vis-à-vis health of human kind.

    Time:

    Title: Conjugated Linoleic Acid, Produced by Anaerobic Gut Fungi, Inhibits the Salmonellosis

    Rabar M. Abdulrahman
    Kahramanmaras Sutcu Imam University, Turkey

    Biography
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    Biography

    Rabar M. Abdulrahman
    Kahramanmaras Sutcu Imam University, Turkey



    Abstract
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    Abstract

    Rabar M. Abdulrahman
    Kahramanmaras Sutcu Imam University, Turkey

    During the last three decades, the epidemiology of foodborne pathogens has changed rapidly along with the alterations in the social atmosphere and the ability of pathogens to adapt to new niches. Food is a source of transmitting diseases through which more than 200 diseases are transmitted .Salmonella is the most commonly suggested cause of foodborne disease which constitutes a major public health problem in many countries. In this study, anaerobic gut fungi (AGF) were isolated from the feacal samples of ruminant herbivores and putatively identified them as Orpinomyces sp. and Neocallimastix species. AGF isolates grown in anaerobic medium containing linoleic acid as sole energy source, to understand whether AGF play a role in the formation of conjugated linoleic acid (CLA) from linoleic acid (LA), and our preliminary findings showed that isolated AGF have ability to convert LA to CLA effectively. The produced CLA was investigated for its possible antibacterial effects against Salmonella. The results showed that CLA formed by one of AGF isolates (Orpinomyces sp. GMLF157) has a remarkable antibacterial effect on all four strains of Salmonella sp. (Salmonella enterica, Salmonella typhi ATCC 14081, Salmonella sp.1 and Salmonella sp.2) used for current study.

    Sessions:
    Workshop

    Time:

    Title: Personalised Nutrition & the Microbiome

    Dr. Suzan Wopereis, Prof. Dr. Bart Keijser, Nard Clabbers
    TNO, Netherlands

    Biography
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    Biography

    Dr. Suzan Wopereis, Prof. Dr. Bart Keijser, Nard Clabbers
    TNO, Netherlands



    Abstract
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    Abstract

    Dr. Suzan Wopereis, Prof. Dr. Bart Keijser, Nard Clabbers
    TNO, Netherlands

  • Sessions:
    Role of Probiotics in Athletes & Sports Nutrition & Probiotics and Bioactive Peptide & Probiotics for Oral Health and Clinical Studies

    Time:

    Title: Thirty Days of Multi-Strain Probiotic Supplementation was Associated with Reduced Incidence of Post-Prandial Dietary Endotoxin, Triglycerides, and Disease Risk Biomarkers

    Thomas Bayne
    Microbiome Labs, USA

    Biography
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    Biography

    Thomas Bayne
    Microbiome Labs, USA

    Tom is a chiropractic physician who specializes in nutritional therapies and is an international expert in digestive health and detoxification. His philosophy addresses the relationship between structure and function of the human body and how that translates into your best health. Tom has over 20 years of experience in natural health and medicine. Tom’s passion for holistic medicine led him to Europe, where he had executive roles at two top natural health companies. As international marketing director for one of Europe’s leading food supplement manufacturers, Dr. Bayne’s responsibilities included formulating supplements based on specific patient conditions, lecturing on his clinical experiences, research that supported natural medicines and developing educational materials for physicians and pharmacies. Tom has developed over 35 highly successful and effective products that are sold directly to physicians. Tom’s 20 years of clinical experience combined with his extensive knowledge in product development has led to the development of MegaSporeBiotic®.



    Abstract
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    Abstract

    Thomas Bayne
    Microbiome Labs, USA

    Dietary or metabolic endotoxemia is a condition that affects approximately 1/3 of individuals living in Western society. It is characterized by increased serum endotoxin concentration during the first five hours of the post-prandial period following consumption of a meal with a high-fat, high-calorie content. Long-term repeated dietary endotoxemia may increase the risk of developing a variety of chronic diseases. Of the available treatments, oral probiotic supplementation has been purported to reduce gastrointestinal permeability to endotoxin, which in theory should suppress the dietary endotoxin response. The purpose of this study was to determine if 30-d of oral probiotic supplementation could reduce post-prandial dietary endotoxemia in “responder” subjects. Apparently healthy men and women (N=75) were screened for post-prandial dietary endotoxemia. Subjects whose serum endotoxin concentration increased by at least 5-fold from pre-meal levels at 5-h post-prandial were considered “responders” and were randomized to receive either placebo (rice flour) or multi-strain probiotic supplement (Bacillus indicus (H36), Bacillus subtilis (H58), Bacillus coagulans, and Bacillus licheniformis, and Bacillus clausii)for 30-d. The dietary endotoxemia test was repeated at the conclusion of the supplementation period. Dietary endotoxin (LAL) and triglycerides (enzymatic) were measured using an automated chemistry analyzer. Serum disease risk biomarkers were measured using bead-based multiplex assays as secondary, exploratory measures. Data were statistically analyzed using repeated measures ANOVA and a P < 0.05. We found that probiotic supplementation was associated with a 42% reduction in endotoxin (P=0.011) and 24% reduction in triglyceride (P=0.004) in post-prandial period Placebo subjects presented with a 36% increase in endotoxin and 5% decrease in triglycerides over the same post-prandial period. We also found that probiotic supplementation was associated with significant post-prandial reductions in IL-12p70 (P=0.017), IL-1 (P=0.020), and ghrelin (P=0.017) compared to placebo subjects. The key findings of the present study, were that oral probiotic supplementation reduced responses that were consistent with “leaky gut syndrome” and transient reductions in chronic/metabolic disease risk.

    Time:

    Title: Efficacy of Lactobacillus Rahmnosus CRD11 against DMH Induced Colon Cancer in Rat Model

    Søren Kjærulff
    FermBiotics, Denmark

    Biography
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    Biography

    Søren Kjærulff
    FermBiotics, Denmark

    Søren Kjærulff, CEO of Fermbiotics and CTO Fermentationexperts. He is the responsible for development of fermented plant and seaweed food and pet food products with microbiome modulatory activity. He is responsible for introducing the product to the market, investigate and develop its functionality, identify the regulatory requirements, as well as go-to-market strategies. Former Novozymes senior director of biopharmaR&D responsible of anti-microbial and anti-inflammatory peptides and albumin half-life extension of peptide drugs. He is the former vice president of R&D at Pantheco and Santaris Pharma.



    Abstract
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    Abstract

    Søren Kjærulff
    FermBiotics, Denmark

    FermBiotics have developed several fermented plant and seaweed products for animal and human health. The products consist of prebiotics, probiotics and bioactives from the fermented plant and seaweed material. Metabolomic studies of the products have revealed several very health related metabolites and bioactives. We have demonstrated very good in vitro anti-microbial and anti-inflammatory activities of the fermented material. FermBiotics has performed several intervention studies in animal species and performed microbiome analysis of the gut bacteria and results have shown significant microbiome modulation. An additional pig trial showed a high (58%) reduction of LDL-cholesterol in pigs given 4 % fermented canola meal diet compared with normal diet. FermBiotics will in collaboration with Silkeborg Hospital, Denmark in the EU project Macro Cascade run a randomized double-blinded matched cross trial using a dose of 5 g fermented canola and seaweed material in 2h of 2018. The clinical trial will beperformed in N=80 human patients with mild-moderate state of Inflammatory Bowel Disease (IBD) and/or Spondyloarthropathy (SPA) including both gender.

    Time:

    Title: Bacteriocinogenic Lactobacillus Plantarum Inhibit Adhesion of Gastrointestinal Pathogens onto Extracellular Matrix and Intestinal Cells

    Sandipan Mukherjee
    Indian Institute of Technology Guwahati, India

    Biography
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    Biography

    Sandipan Mukherjee
    Indian Institute of Technology Guwahati, India

    Sandipan Mukherjee is a doctoral student in the Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, India. During his doctoral tenure, he has essentially ascertained the probiotic attributes of native lactic acid bacteria (LAB) and its secreted bacteriocin in inhibiting pathogen adhesion onto extracellular matrix and cultured human intestinal cells.



    Abstract
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    Abstract

    Sandipan Mukherjee
    Indian Institute of Technology Guwahati, India

    Probiotic lactic acid bacteria (LAB) can be explored to develop a safe and niche-specific therapeutic regimen against bacterial infections based on their ability to thwart adhesion of pathogens onto intestinal cells. The present investigation reports the potential of native Lactobacillus plantarum strains to inhibit adhesion of model gastrointestinal pathogens, Enterococcus faecalis and Staphylococcus aureus on extracellular matrix (ECM) and cultured intestinal cells (HT-29 cells). A native L. plantarum CRA21 strain rendered significant inhibition of S. aureus MTCC 96 and E. faecalis MTCC 439 adhesion onto ECM molecules, collagen and mucin, with the highest inhibition of S. aureus MTCC 96 adhered on collagen observed in the exclusion mode (83.61%) as compared to competition (62.4%) and displacement (38.58%) mode of adhesion assay. A dual-color flow cytometry (FCM) based adhesion assay indicated that in the exclusion mode, L. plantarum DF9 rendered notable inhibition of pathogen adhesion onto HT-29 cells, with the relative adhered population of E. faecalis MTCC 439 being 3.94% and that of L. plantarum DF9 being 77.56%. FCM along with principal component analysis (PCA) revealed that the native strains of L. plantarum DF9 and L. plantarum CRA38 could significantly affect both the adhesion process parameters kd and em of pathogen, similar to the standard probiotic L. rhamnosus GG. Interestingly, addition of the bacteriocin plantaricin A obtained from L. plantarum could reduce the viability of ECM-adhered pathogens. Host cell adhesion assays indicated that addition of plantaricin A on LAB and pathogen adhered onto HT-29 cells led to a prominent reduction in the adhered E. faecalis MTCC 439 cells (17.4%) as compared to L. plantarum DF9 (70.5%), which highlighted the benefit of using LAB bacteriocin for selective eradication of pathogen and minimal collateral damage. It is envisaged that the native probiotic L. plantarum strains can be used in conjunction with LAB bacteriocins as niche-specific anti-adhesion therapeutic agents against gastrointestinal pathogens.

    Time:

    Title: Proteomic Characterization of Bioactive Peptide from Endophytic Bacillus cereus

    Rachana Pandey
    Dr. D.Y. Patil Biotechnology & Bioinformatics Institute, India

    Biography
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    Biography

    Rachana Pandey
    Dr. D.Y. Patil Biotechnology & Bioinformatics Institute, India



    Abstract
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    Abstract

    Rachana Pandey
    Dr. D.Y. Patil Biotechnology & Bioinformatics Institute, India

    Bioactive peptides or antimicrobial peptides (AMPs) characterization has received great attention in the recent past due to their application as therapeutic agents and also as food preservatives without any toxic effects on host.At present,food preservation is a serious concern for almost all countries across the worldA serious challenge to antimicrobial therapies is the rapid increase in antibiotic resistant infections. Therefore, an urgent need to develop other biocontrol agents is the need of the hour.Staphylococcus aureus is a major bacterial pathogen that causes clinical infection and foodborne illnesses. Food preservatives are widely used to reduce the risk of food poisoning. With growing consumer demand for natural preservatives to replace chemical compounds, new antimicrobial products of various origins are being developed.Endophytes from enormous number of diverse plants and environmental conditions are potential source for isolation of bioactive compounds.While a wide range of biologically active compounds have been isolated from endophytic organisms, they still remain a relatively untapped source of novel natural product. Endophytic microbes seem to fit perfectly into this natural warehouse, only a small part of which we have been able to tap in so far. In this study, aBacillus strain showed antimicrobial activity characterized by Kirby Bauer method of well/ disc diffusion and soft agar overlay assay. The bacterial supernatant showed zone of inhibition after 16hr of incubation at 370C against Staphylococcus. The culture supernatant was precipitated with ammoniumsulphate (80% saturation) and column chromatography was performed with Sephadex G-50 followed by DEAE column purification. The purified fraction was used for characterization by Sodium Dodecyl–Polyacrylamide Gel electrophoresis (SDS –PAGE). The protein band thus resolved was taken for analysis by mass spectrometry (MALDI-TOF).Peptide mass fingerprint Analysis with MALDI showed bioactivepeptide of low molecular weight.

    Time:

    Title: The functional microbiome and the biofilm phenotypes in human microbiome discovery

    Nicholas T. Monsul
    Quorum Innovations, USA

    Biography
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    Biography

    Nicholas T. Monsul
    Quorum Innovations, USA

    Dr. Nicholas Monsul trained as a physician and surgeon at Yale University School of Medicine and The Johns Hospital where he was involved in performing surgery, conducting basic science research and teaching residents. He Co-Founder Quorum Innovations, a microbiome based therapeutics research and development company, where he is co-inventor on 7 issued United States patents.



    Abstract
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    Abstract

    Nicholas T. Monsul
    Quorum Innovations, USA

    The clinical relevance of microbial biofilm phenotypes – sessile microbial communities characterized by genes in gene expression and which secrete extracellular polymeric substances – has been underestimated in the disease treatment and microbiome drug discovery paradigms. Ubiquitous in nature and quite likely the “preferred form of life” for microbes due to the inherent survival benefits conferred by the biofilm phenotype, these polymicrobial populations function as a unit and are found on virtually every internal and external human body surface. Microbiota in biofilm phenotype are species specific, hard to detect, and don’t fit into a developed diagnostic or regulatory rubric. Yet, could they be our “microbiome stem cells”, survivalists protected in gel-like biofilms and differential gene expression which dynamically seed local human tissues? Are bacteria in a biofilm phenotype a critical untapped discovery source? And could microbial biofilms explain some unanswered questions of human health and disease – clinically apparent infections but negative culture results, unexplainable chronic inflammation, auto-immunity? This talk seeks to acquaint the listener with some conceptual framework behind human mucosal and epidermal microbiota in biofilms and the functional microbiome and their relationships to human disease and their potential applications to human microbiome drug discovery.

    Time:

    Title: Periodontal Muscle Training Can Strength the Periodontal Support Feet Your Teeth

    Nima Sabzchamanara
    National Medical University Kiev, Ukraine

    Biography
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    Biography

    Nima Sabzchamanara
    National Medical University Kiev, Ukraine

    Nima Sabzchamanara has completed his dental study from National Medical University Kiev, Ukraine. He is the student of first year of Residency program, in the Department for Therapeutic dentistry. He has published one abstract in a dental journal.



    Abstract
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    Abstract

    Nima Sabzchamanara
    National Medical University Kiev, Ukraine

    Previous research on periodontal structure and function has shown a significant relationship between periodontal tissue and teeth. This study assessed dentist's beliefs about the relative efficacy of the health of periodontal tissue. A total of 505 patients in general pracrice were asked to respond to a list of 25 obligatory nourishment for a child while going to have the first teeth, for its effectiveness in dealing with patient's periodontal health especially include chewing hard food. They were also asked to select the three most effective nutrition for periodontal tissue. The imdices of patient perceived importance of the periodontal health were derived and each compared with actual effectiveness as determined from a sample of 250 patients. Although the majority of patient's rated 18 of 25 nutrition as being very effective, there was no significant association between patient perceived nourishment effectiveness and actual effectiveness. The implications for patient training are discussed. This study supported by only me and my supervisor Alla grigorivna demitrova.

    Time:

    Title: Synbiotic-Assisted Modulation of Gastrointestinal Microbiota and its Effect on Human Health

    C. D. Khedkar
    Maharashtra Animal & Fishery Sciences University, India

    Biography
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    Biography

    C. D. Khedkar
    Maharashtra Animal & Fishery Sciences University, India

    Dr. CD Khedkar is Associate Professor and Head, Deptt. of Dairy Microbiology and Biotechnology. He has two doctoral degrees to his credit, one in Microbiology and the other in Dairy Biotechnology. He has 27 years experience of teaching, research and extension. His field of specialization is in probiotics, prebiotics and value-addition of dairy products/food commodities. Till today eight doctoral and 15 postgraduate students have successfully completed their degrees under his guidance. He is the brain behind the development of a world-bank funded consortia-mode project aimed at sustainable rural livelihood security through integrated approaches. He attended several scientific conferences/seminars in India and abroad in the capacity as the Chairman/Lead Speaker. He has more than 150 scientific publications to his credit. He is bestowed with several awards/recognitions for his scientific excellence and academic and extension activities to his credit.



    Abstract
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    Abstract

    C. D. Khedkar
    Maharashtra Animal & Fishery Sciences University, India

    The growing concern with food habits and their relation to health prompted several studies in food science. The gastro-intestinal (GIT) microbiota respond to anatomical and physicochemical variations in the tract. This microbiota comprises of pathogenic, benevolent and advantageous. The composition and activity of the GIT develop with the host from birth and it depends on nutrition and life-style. It is involved in regulating metabolic pathways and physiologically connect the gut, liver, muscle, and brain. It is evident that the gut microbiota can be favourably modulated by synbiotic interventions to combat diseases and improve the human health. In recent years, the concept of functional foods has been emerged to examine food additives that may exert beneficial effects on the GIT microbiota. A most prominent class of functional foods in focus is synbiotics, which is a suitable combination of probiotics and prebiotics. They exert beneficial effects through four main mechanisms i.e. immunomodulation, interference with potential pathogens, improvement of barrier function and production of neurotransmitters. Their host targets vary from the resident microbiota to cellular components of the gut-brain axis. Synbiotics are now being considered important tools to help maintain humans and animals in good health and in prevention and/or alternatives for reducing the risk associated to a number of diseases. However, relatively few effects have been supported by a battery of data from clinical trials. Owing to the fact malnourished children struggle to gain weight even on high-nutrient diets, a long-term feeding trial comprising of 80 tribal children of 2-5 years with synbiotics was conducted for its suitability to combat malnutrition-led health effects. It was revealed that the feeding of synbiotics @200 ml per child for 90 days exerted a positive effect on health parameters. It is concluded that regular feeding of synbiotics considerably reduce the risk of malnutrition-led mortality of tribal kids faced by developing countries.

    Sessions:
    Poster Competition

    Time:

    Title: Characterization of Bacteriocin from Indigenous Probiotic Lactobacillus with Emphasis on Broad Antibacterial Spectrum

    Debashis Halder
    University of Gour Banga, India

    Biography
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    Biography

    Debashis Halder
    University of Gour Banga, India

    Debashis Halder is involved in Ph. D Research since last three years, under the supervision of Prof. Shyamapada Mandal, Laboratory of Microbiology and Experimental Medicine, Department of Zoology, University of GourBanga, India. He has worked on three projects and published five research articles in four reputed international journals. He is passionate to pursue a career in Medical Microbiology and truly attracted by Molecular Immunology and Phytomedicine. Hewishes to continue as a scientist with a dynamic team of sincere researchers along with continuous research in his fields and extend his valuable service towards the scientific community with extensive research work.



    Abstract
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    Abstract

    Debashis Halder
    University of Gour Banga, India

    Background: Bacteriocinsfrom lactic acid bacteria (LAB) have been excellent biotheraputic agents to be used against pathogenic bacteria. However, the antibacterial activities of bacteriocins from indigenous LAB have not been validated scientifically from our part of the globe. The current study determines the antibacterial properties and characterize bacteriocin isolated from local Lactobacillus strains. Methods: Five commercial curd samples were processed microbiologically, using MRS media, in order to isolate LAB. The LAB were identified following conventionaland molecular methods, and the probiotic features were validated. Antagonistic property of the isolated LAB were determined against the indicator pathogenic bacteria. Bacteriocin isolated from such probiotic strains were characterized through enzyme treatment, SDS-PAGE analysis and antibacterial property. Result: The LAB isolates were identified as Lactobacillus Spp. and the isolates showed probiotic features, with tolerance to variable temperature (15°C and 42°C) and acid (pH 2-4), bile salt (0.125%, 0.25% and 0.50 %) and NaCl (2%, 4% and 6.5%). The LAB were γ-hemolytic and did not cause gelatin hydrolysis and mostly were sensitive to the antibiotics tested. Antagonistic activity test revelsa zone diameter of inhibition (ZDI) of 16-46 mm and 7-26 mm in agar-overlay and agar-well techniques, respectively, against the gram positive and gram negative indicator bacterial pathogens. The isolated bacteriocin from probiotic LAB were of 11-14 kDa. The bacteriocin showed antibacterial activity displaying ZDIs 15-30 mm and minimum inhibitory concentration (MICs) 7.12 – 483.12 µg/ml. Conclusion: The lactobacilli were excellent probiotics, and thebacteriocin from the LAB had a broad spectrum of antibacterial activity. Thus, the indigenous Lactobacillus strains from local niches and the bacteriocin isolated from them might be used as biotherapeutic agents against bacterial infection.

    Time:

    Title: Use of Encapsulated B. Lactis through Freeze-Drying and Extrusion for Probiotic Yogurt Production

    Konstantinos Alexias
    Technical University of Delft, Netherlands

    Biography
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    Biography

    Konstantinos Alexias
    Technical University of Delft, Netherlands



    Abstract
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    Abstract

    Konstantinos Alexias
    Technical University of Delft, Netherlands

    Time:

    Title: Indigenous Probiotic Lactobacillus Isolates Presenting Antibiotic like Activity against Human Pathogenic Bacteria

    Debashis Halder
    University of Gour Banga, India

    Biography
    χ

    Biography

    Debashis Halder
    University of Gour Banga, India

    Debashis Halder is involved in Ph. D Research since last three years, under the supervision of Prof. ShyamapadaMandal, Laboratory of Microbiology and Experimental Medicine, Department of Zoology, University of GourBanga, India. He has worked on three projects and published five research articles in four reputed international journals. He is passionate to pursue a career in Medical Microbiology and truly attracted by Molecular Immunology and Phytomedicine. Hewishes to continue as a scientist with a dynamic team of sincere researchers along with continuous research in his fields and extend his valuable service towards the scientific community with extensive research work.



    Abstract
    χ

    Abstract

    Debashis Halder
    University of Gour Banga, India

    Background: Indigenous lactic acid bacteria are well known probiotics having antibacterialactivity against potentially pathogenic bacteria. This study aims to characterize the curd lactobacilli for their probiotic potentiality and antagonistic activity against clinical bacteria. Methods: Four curd samples were processed microbiologically for the isolation of lactic acid bacteria (LAB). The LAB strains obtained were identified by conventional methods: cultural aspect, gram-staining, biochemical and sugar fermentation tests. The probiotic properties were justified with tolerance to low-pH, bile salt and sodium chloride, and the antagonistic activity of the lactobacilli against human pathogenic bacteria (Escherichia coli, Proteus vulgaris, Acinetobacterbaumanniiand Salmonella entericaserovarTyphi) was assessed. Hemolytic activity and antibiotic susceptibility were determined for the lactobacilli isolates, and the cumulative probiotic potential (CPP) values were recorded. Result: Four lactobacilli isolates, L. animalisLMEM6, L. plantarumLMEM7, L. acidophilusLMEM8 and L. rhamnosusLMEM9, procured from the curd samples, survived in low-pH and high bile salt conditions, and showed growth inhibitory activity against the indicator bacteria by agar-well (zone diameter of inhibition; ZDIs: 13.67 ±0.58–29.50 ±2.10 mm) and agar overlay (ZDIs: 11.33 ±0.58–35.67 ±2.52 mm) methods; the average growth inhibitory activity of lactobacilli ranged 233.34 ±45.54–280.56 ±83.67 AU/mL, against the test bacterial pathogens. All the lactobacilli were non-hemolytic and sensitive to most of the test antibiotics. The CPP values of the isolated LAB were recorded as 80–100%. Conclusion: The curd lactobacilli procured might be used as the valid candidates of probiotics, and bio-therapeutics against bacterial infection to humans.

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