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International
Probiotics and Antimicrobial Proteins Conference

November 6-8, 2017 | Barcelona, Spain

Program Schedule

  • Sessions:
    Dairy technology & Industrial production and microbial fermentation

    Time:

    Title: The two-in-one use of sweet whey affords yet unknown probiotic viability upon drying

    Gwenael Jan
    INRA-Agrocampus West Rennes, Science and Technology of Milk and Egg, France

    Biography
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    Biography

    Gwenael Jan
    INRA-Agrocampus West Rennes, Science and Technology of Milk and Egg, France



    Abstract
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    Abstract

    Gwenael Jan
    INRA-Agrocampus West Rennes, Science and Technology of Milk and Egg, France

    Introduction: Probiotic efficacyrelies on administration of live and active probiotic strains in adequate dose.Growth yield and stress toleranceduring probiotic production and deliverythus constitute a keybottleneck. Probiotics are widely produced, stored and used under a dried form, mainly by freeze-drying. Drying process generally comprises culture, harvesting, washing and drying steps in which preservation of viability remains a quest for the Holy Grail. Methods: The aqueous phase of fermented dairy products was shown to enhance stress tolerance in both lactic and propionic acid bacteria. We used sweet whey, a dairy industrybyproduct, as a two-in-one medium to sustain both growthof probiotics, and then directly spray-drying without harvesting and washing steps. Moreover, hyperconcentrated sweet whey was developed to achieve one-step drying with higher level of dry matter. Results: Both lactic and propionic acid bacteria were adapted to growth within sweet whey and resulting cultures were directly spray-dried with various survival rates, depending on dairy components concentration. Interestingly,growth of probiotics in hyperconcentrated sweet whey led to enhanced stress tolerance, overexpression of key stress proteins, accumulation of intracellular storage molecules and compatible solutes, consequently resulting in yet unknown survival upon heat, acid and bile challenges, as well as spray-drying and storage. Discussion: Spray- being far more cost-effective than freeze-drying, this innovation opens new avenues for sustainable development of probiotic products with enhanced delivery efficiency. This patent-protected new process indeed uses a dairy industry byproduct, requires limited amounts of energy, affords high bacterial viability and protects probiotics from injury undergone within the digestive tract.

    Time:

    Title: Grape marc is a functional substrate for probiotic bacteria

    Maria De Angelis
    University of Bari Aldo Moro, Italy

    Biography
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    Biography

    Maria De Angelis
    University of Bari Aldo Moro, Italy



    Abstract
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    Abstract

    Maria De Angelis
    University of Bari Aldo Moro, Italy

    This study aimed at using grape marc for the growth of probiotics with the perspective of producing a functional ingredient having antioxidant activity. Lactobacillus plantarum 12Aand PU1, Lactobacillus paracasei 14A, and Bifidobacteriumbreve 15A showed the ability to grow on grapemarc (GM) based media. The highest bacterial cell density (>9.0 CFU/g) was found in GM added of 1% ofglucose (GMG). Compared to un-inoculated and incubated control fermented GMG showed a decrease ofcarbohydrates and citric acid together with an increase of lactic acid. The content of several free aminoacids and phenol compounds differed between samples. Based on the survival under simulated gastrointestinalconditions, GMG was a suitable carrier of lactic acid bacteria and bifidobacteria strains. Compared to the control, cell-free supernatant (CFS) of fermented GMG exhibited a marked antioxidantactivity in vitro. The increased antioxidant activity was confirmed using Caco-2 cell line after inducingoxidative stress, and determining cell viability and radical scavenging activity through MTT and DCFH-DAassays, respectively. Supporting these founding, the SOD-2 gene expression of Caco-2 cells also showed alowest pro-oxidant effect induced by the four CFS of GMG fermented by probiotic strains.

    Time:

    Title: Physicochemical and Microbiological Properties of Milk Jiaokoufrom Inner Mongolia: Incredible Diversity of Nutritional Ingredients

    Wenli Liu
    College of Chemistry and Environmental Engineering, Shenzhen University, China

    Biography
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    Biography

    Wenli Liu
    College of Chemistry and Environmental Engineering, Shenzhen University, China



    Abstract
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    Abstract

    Wenli Liu
    College of Chemistry and Environmental Engineering, Shenzhen University, China

    Milk Jiaokou is a traditional and handmade fermented milk product generally manufactured by herdsmensin Inner Mongolia of China. Usually, it is made from cow, sheep, horse, or camel milk, individually. To date, this product is still used as a zest dish for the herdsmen s groups in some Inner Mongolia regions. According to the history book Milk Jiaokou has abundant nutritional values and many physiological functions, but Milk Jiaokouhas not yet deep studied or commercially developed in China and other countries so that it is poorly understood. In order to further study Milk Jiaokou its nutrient ingredients, chemical and physical properties and microbiology analysis were made using analysis experiment and plate count method in this study. The results revealed that their solid contents of Milk Jiaokou from the herdsmen of XinlinGol League, Lanqi,Tongliao city and Turehot of Chifengrespectively were high,90.36±0.65g/100g, 92.21±0.28g/100g, 90.24±0.08g/100g, and 92.18±0.38g/100g respectively. The pH values were acidic, 3.25±0.01, 3.68±0.01, 4.31±0.01, 3.82±0.01, sequentially. The protein contents were a little low, 1.76%, 2.10%, 1.53%, and 1.98%, respectively. The total sugar contents were 55mg/g, 39.9mg/g, 52.9mg/gand 70mg/g. Amino acids were abundant, their total amino acid content were1.59g/100g (wherein Asp, Glu, Val, Leu, Phe and Lys were important amino acid), 1.31g/100g (wherein Asp, Glu, Val, Leu, Phe and Lys were important amino acid), 1.51g/100g (wherein Asp, Glu, Pro, Val, Leu and Lys were important amino acid), and 1.51g/100g (Glu, Pro and Leu were important amino acid), respectively. The Milk Jiaokou had abundant microbiological resources which were mostly comprised of Lactobacillus, acetic acid bacteria, yeast, and more. Similarly, the range of the total bacterial counts were from 7.04E+07±0.01E+07 to 8.45E+07±0.21E+07. The colony forming units (CFU) of Lactobacillus in each sample were 1.55E+05±0.15E+05, 1.70E+05±0.10E+05, 2.35E+05±0.35E+05, and 2.30E+05±0.50E+05. The CFU for the acetic acid bacteria were 1.85E+06±0.11E+06, 1.66E+06±0.10E+06, 1.72E+06±0.12E+06, and 1.86E+06±0.23E+06, respectively. The CFU for yeast were 1.03E+07±0.03E+07, 7.73E+06±0.37E+06, 7.72E+06±0.23E+06, and 9.58E+06±1.99E+06, respectively. In conclusion, Milk Jiaokou had abundant nutritional values and good physical and chemical properties, which will establish the basis for deeply development of its physiological functions.

    Time:

    Title: Design of experiment approach for developing probiotic and prebiotic fortified fermented food product

    Bijender Kumar
    University of Jammu, India

    Biography
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    Biography

    Bijender Kumar
    University of Jammu, India



    Abstract
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    Abstract

    Bijender Kumar
    University of Jammu, India

    Human gut microbiota plays a significant role in determining health and disease status. Probiotics may help maintaining favorable composition of gut microbiota, and can even reverse the ill effects of dysbiosis thus, contributing towards sustaining good health. Several of the potential health benefits of probiotics are well established, and numerous are still under investigation, and wanting more evidences. Probiotics show immense diversity with respect to their health benefitting attributes i.e. health benefits earmarked in one strain/species cannot be extrapolated to others without active experimentation. Thus, bioprospecting of novel efficacious probiotic strains is a continuous practice. Dairy products have widely been used as probiotic vehicles, however, recently earned some criticism due to high fat content, lactose intolerance, and allergies. Therefore, alternative probiotics-vehicles are being explored. Cereals could be one of the good choices as probiotic carriers. The current study aimed at isolating efficient probiotics, their functional characterization, and application for development of oat-based fermented food product using design of experiment (DOE) approach.

    Time:

    Title: Electrohydrodynamic processing for probiotic encapsulation

    Lorenzo Pastrana
    INL - International Iberian Nanotechnology Laboratory, Portugual

    Biography
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    Biography

    Lorenzo Pastrana
    INL - International Iberian Nanotechnology Laboratory, Portugual

    Prof. Lorenzo Pastrana has a PhD in Pharmacy (1991) by the University of Santiago (Spain). He was postdoctoral fellow (1992-1993) in the Centre de Transfert en Microbiologie et Biotechnologie INSA at Toulouse (France). In 1991 he joined the University of Vigo and since 2010 is Professor of Food Science. He founded the Galician Agri-Food Technology Platform (2006). He worked in pre production of bacteriocins and probiotics as well as its applications in food industry. Currently he is working in the production of nanostructures with food grade polymers for controlled or mucoadhesive release of bioactives in food packaging/processing applications. In September 2015 he joined the INL as Head of the Department of Life Sciences.



    Abstract
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    Abstract

    Lorenzo Pastrana
    INL - International Iberian Nanotechnology Laboratory, Portugual

    Electrohydrodynamic processing has been recently suggested to be a simple and straightforward method to generate micro- and nanostructures and pointed as an emerging technology, which can be employed as a tool for the encapsulation of functional ingredients. Some of its advantages are: no requirement of high temperatures (i.e. sensitive ingredients can be encapsulated without any activity loss) and the possibility of using only water-based solutions.Electrohydrodynamic processes such as: electrospinning and electrosprayingwere used for the production of bio-based structures systems for probioticsencapsulation, that can be then used in food and pharmaceutical applications. The two processes are very effective to produce bio-based nanostructures with several advantages that overcome the existing technology limitations, such as: different sizes range (micro to nano) and type of structures (fibres and particles), no temperature required, ability to scale-up (multi-nozzle systems), versatility in morphology (porosity and roughness) and low or no organic solvents requirement. Moreover, they guarantee high encapsulation efficiencies, a good stability and a possible controlled release. Several materials were tested (whey protein isolate, ethylcellulose, zein) to produce those structures, being possible to obtain fibres and particles according to the solutions conditions (concentration of biopolymer and ethanol concentration) and processing conditions (voltage and needle diameter). Results showed that whey protein isolate is the most effective material to encapsulate probiotics, being possible to obtain capsules with sizes ranged between 5 and 0.5 µm. The encapsulation efficiency was 100% being the probiotics stable after processing. The probiotics stability was performed during 3 months showing good results when compared with freeze-dried probiotics. Electrohydrodynamic processes showed to be a good option to replace spray-drying and freeze-drying technologies for the encapsulation of probiotics.

    Time:

    Title: In vitro investigation of anticancer, antihypertensive, antidiabetic, and antioxidant activities of camel milk fermented with camel milk’s probiotic: A comparative study with fermented bovine milk

    Mutamed Ayyasha
    United Arab Emirates University, UAE

    Biography
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    Biography

    Mutamed Ayyasha
    United Arab Emirates University, UAE

    Dr. Mutamed Ayyash is an Assistant Professor of Food Microbiology and Safety in the Department of food Science at UAE University. He teaches advance courses in food science and statistical analysis for master students. Dr. Ayyash also leads the research group in food microbiology area and coordinates the master program of food science. Dr. Ayyash completed his BSc degree in Agriculture Engineer major in Food Science in 2002 from Damascus University. Afterwards, Dr Ayyash worked for two years in food industry sector which highly encouraged him to pursue his postgraduate studies. Dr. Ayyash joined the master program of food science at Jordan University of Science and Technology and completed his MSc degree in 2007. During his MSc study, Dr. Ayyash worked hardly on foodborne pathogens area with a research group at food science department. Outcomes of the research group have been published in referred international journals. Dr. Ayyash completed his PhD at Victoria University, Australia in 2013.



    Abstract
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    Abstract

    Mutamed Ayyasha
    United Arab Emirates University, UAE

    Objective: the aim of this study was to in-vitro investigate antiproliferation activity, ⍺-amylase and ⍺-glucosidase inhibitions, antioxidant, ACE-inhibition, and proteolytic activities of camel milk fermented with Lactococcus lactis KX881782 (Lc.K782) compared with fermented bovine milk during 21 days of storage at 4 C. Lactobacillus acidophilus as a well-regarded probiotic will be employed as a control. Method: Both milk types were pasteurized at 90 C for 10 min followed by cooling at 37 C. Pasteurized milk was inoculated with 1% (w/v) of each strain separately (~ 9.0 log10 CFU/mL), followed by mixing for 1 min. Inoculated milk was fermented at 37 C for 24 h. Afterward, fermented milk was cooled to 4 C in ice-bath, then stored at 4 C for 21 days. Fermented milk was sampled at 0, 7, 14 and 21 day of storage. Results: The proteolytic activities of water-soluble extract (WSE) in all fermented camel milk were higher than those of fermented bovine milk. The α-glucosidase inhibitions in both milk types fermented by Lc.K782 ranged from 30 – 40%. Camel milk fermented by Lc.K782 had the highest antioxidant by ABTS. The highest ACE-inhibition of WSE in camel milk fermented by Lc.K782 was > 80%. The proliferations of Caco-2, MCF-7 and HELA cells were more inhibited when treated with WSE of fermented camel milk extracts. Conclusions: This study showed that health-promoting benefits particularly antioxidant, ACE-inhibition and antiproliferative activities of WSE in fermented camel milk were markedly higher than those in fermented bovine milk. Moreover, the WSE in camel milk fermented by indigenous lactic acid bacteria Lc.K782 exhibited greater potential health benefits than non-indigenous strains (La.DSM).

    Sessions:
    Gut microbiome and microbiota & Probiotics in Food Technology and Health Promotion

    Time:

    Title: Combining selected immunomodulatory Propionibacterium freudenreichii and Lactobacillus delbrueckii strains: reverse engineering development of an anti-inflammatory cheese

    Gwenael Jan
    INRA-Agrocampus West Rennes, Science and Technology of Milk and Egg, France

    Biography
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    Biography

    Gwenael Jan
    INRA-Agrocampus West Rennes, Science and Technology of Milk and Egg, France



    Abstract
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    Abstract

    Gwenael Jan
    INRA-Agrocampus West Rennes, Science and Technology of Milk and Egg, France

    Combining selected immunomodulatory Propionibacterium freudenreichii and Lactobacillus delbrueckii strains: reverse engineering development of an anti-inflammatory cheese. Introduction Inflammatory bowel disease (IBD) constitute a growing public health concern in western countries. More generally, inflammation is a key parameter involved in many non-communicable chronic diseases. Bacteria with anti-inflammatory properties are lacking in the dysbiosis accompanying IBD. Selected strains of probiotic bacteria with anti-inflammatory properties accordingly alleviate symptoms and enhance treatment of ulcerative colitis in clinical trials. Such properties are also found in (only) selected strains of dairy starters, including thePropionibacterium freudenreichii and Lactobacillus delbrueckii species, which are widely ingested. We thus investigated the possibility to develop a fermented dairy product, combining both starter and probiotic abilities of both lactic acid and propionic acid bacteria, designed to extend remissions in IBD patients. Methods We developed a single-strain L. delbrueckii-fermented milk and a two-strain P. freudenreichii and L. delbrueckii-fermented experimental pressed cheese using strains previously selected for their anti-inflammatory properties. This cheese was made in fully mastered conditions and contained only the two selected strains. It was consumed daily by mice during five days before induction of acute colitis by trinitrobenzenesulfonicacid (TNBS). Outcomes of colitis induction were then monitored in mice consuming this cheese or a placebo diary matrix. Results For the first time, consumption of a designer P. freudenreichii and Lactobacillus delbrueckii pressed cheese was shown to protect mice against experimental colitis, alleviating severity of symptoms, modulating local and systemic inflammation, as well as colonic oxidative stress and epithelial cell damages. As a control, the corresponding sterile dairy matrix failed to afford such protection. Discussion We eat more bacteria through daily consumption of fermented dairy products then through our consumption of functional food supplements. This offers a tremendous level to modulate the digestive tract through the intake of desired bacteria. This work reveals the probiotic potential of a mixture of lactic and propionic selected starters, in the context of fermented dairy products. A “two-in-one” ability is revealed for bacteria able both to produce a fermented dairy product and to modulate inflammation. Such an innovation opens new avenues for the reverse engineering development of anti-inflammatory fermented foods designed for target populations with IBD, and has provided evidences leading to an ongoing pilot clinical study in ulcerative colitis patients.

    Time:

    Title: Probiotics increase amlodipine absorption: an in vivo study in rabbits

    Febrina A. Saputri
    Universitas Padjadjaran, Indonesia.

    Biography
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    Biography

    Febrina A. Saputri
    Universitas Padjadjaran, Indonesia.



    Abstract
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    Abstract

    Febrina A. Saputri
    Universitas Padjadjaran, Indonesia.

    Probiotics may play key roles in human health through its metabolic activities and physiological regulation, including as promoter of nutrient absorption. Therefore, regular consumption of probiotics may increase the absorption of nutrient and drug consumed. In this study, we investigated the effect of probiotic on plasma amlodipine, a dihydropyridine calcium antagonist for the treatment of angina and hypertension, as the model of drug absorption. 18 male New Zealand rabbits were randomly allocated to 3 groups: control group, and two probiotics supplemented groups (Lactobacillus plantarum-strained and Enterococcus faecium-strained, respectively). Probiotics were supplemented for 14 consecutive days before administration of amlodipine, followed by blood collection at various time. Amlodipine concentration was analyzed using a reversed-phase liquid chromatography with fluoresence detector. The results showed that amlodipine concentration of probiotic supplemented groups were significantly higher than the control group. This may provide evidence of the benefit of probiotics consumption during long-term pharmacotherapy.

    Time:

    Title: A Meta-Analysis To Establish The Effects Of Probiotics On Growth Performance And Diarrhea Reduction In Swine

    Marilen P. Balolong
    University of the Philippines, Philippines

    Biography
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    Biography

    Marilen P. Balolong
    University of the Philippines, Philippines

    Dr. Marilen Parungao Balolong, is an Associate Professor at University of the Philippines Manila, Philippines. Her appreciation for Microbiology started during her undergraduate years when she took up BS Biology at UPLB (1995). She further honed her skills when she took MS Microbiology, minor in Biochemistry, at UPLB (2002) and her doctorate degree, DrPH Medical Microbiology, at UP Manila (2015). She completed her post Doctoral at Dankook University, Republic of Korea. Dr. Balolong is one of the recently listed top scientists of the University of the Philippines. Her well-cited works are published in international journals. Dr. Balolong is also a recipient of international awards in Thailand and Indonesia for her work on Probiotics and Functional Foods to Understand and Manage Obesity and Blood Glucose Levels. She is currently professionally affiliated with the following national and international societies: American Society for Microbiology, Korean Society for Microbiology and Biotechnology, Philippine Society for Lactic Acid Bacteria; Philippine Association for the Advancement of Science; Philippine Society for Microbiology, Inc.; Philippine Academy of Microbiology; Mycological Society of the Philippines; Philippine Network of Microbial Culture Collections.



    Abstract
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    Abstract

    Marilen P. Balolong
    University of the Philippines, Philippines

    We conducted a meta-analysis to evaluate the effects of probioticson the growth performance and reduction of diarrhea in swine. We searched databases (e.g., PubMed, Scopus, and EMBase [ScienceDirect]) for papers written in English from 2005 to 2016. The inclusion criteria were as follows: studies using randomized and controlled experimental designswith pigs at any stage of development, with or without a pathogen challenge, published in peer-reviewed journals. Twenty six papers covering more than 800 experiments were considered for evaluation to establish the effects of probiotics on growth performance as measured by average daily gain (ADG), average daily feed intake (ADFI) and feed efficiency (FE). In addition,probiotic effects on diarrhea incidence (DI) and total coliform population (TC) and E. coli (EC) were also assessed.Probiotic supplementation of feeds increased ADG (mean difference = 23.4 g/day) and has improved feed efficiency (mean difference = −0.0188 kg feed/kg body weight). Although antibiotic supplementation contributed to more gain ADG (mean difference = 39.3 g/day), feed efficiency from administered probiotics was better (mean difference = 0.0313 kg feed/kg body weight). Mono-strain probiotics in feeds also favored growth performance ADG (mean difference = 21.4 g/day)and feed efficiency (mean difference = −0.0504 kg feed/kg body weight) than multi-strain probiotics with ADG (mean difference = 19.7 g/day) and (mean difference = −0.0037 kg feed/kg body weight), respectively,when added to feeds. Effects of probiotics on daily gain were more prominent in piglets but feed efficiency was more noticeable in adult pigs. Mono-strain probiotics were more effective in significantly reducing DIand TCs, whereas multi-strain probiotic preparations were effective in reducing EC. Probiotic supplementation also significantly reduced DI and TCs in adult pigs as well as EC in piglets. The magnitude of probiotic effects was similar to that of antibiotics in reducing DI when added to feed, regardless of the age of the pigs. Additionally, probiotics performed similarly to antibiotics in experiments using mono-strain and adult pigs to reduceTCs. We observed significant evidence of inter-experiment heterogeneity, which may have resulted from differences in study designs among experiments or from other factors not considered in our analysis. Overall, our results affirm the benefits of probiotic administration in swine for improved growth performance and reducing diarrhea.

    Time:

    Title: Differences and similarities of immune responses underlying allergy improvements following supplementation of Lactobacillus plantarum IS-10506 (LIS) between allergic children and allergic modeled Balb/c mice

    CRS Prakoeswa
    UniversitasAirlangga, Indonesia

    Biography
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    Biography

    CRS Prakoeswa
    UniversitasAirlangga, Indonesia

    Dr. Cita Rosita Sigit Prakoeswa has 15 years of working experience in Department of Dermatology and Venereology, Faculty of Medicine, UniversitasAirlangga - Dr Soetomo Teaching Hospital. Since 2016, Dr. Prakoeswa heads the Research and Development Board of Dr. Soetomo Teaching Hospital and since 2009 as an Expert Panel in the Ministry of Health (Risbiniptekdok), Indonesia. She was awardedwith four international awards (two best presenter awards and two scholarship awards) and also High Achievement Lecturer at UniversitasAirlangga. Dissemination ofvarious results of her researches carried out through books, journals, and presentation in scientific meetings.



    Abstract
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    Abstract

    CRS Prakoeswa
    UniversitasAirlangga, Indonesia

    Previous research showed potential role of probiotics for allergy, but the mechanism is still unclear. This study aimed to determine the differences and similarities of immune responses for suppressing allergy after supplementation of Lactobacillusplantarum IS-10506 of dadih origin, between allergic children and allergic modeled Balb/c mice. The study was conducted in 2 steps with two types of subjects, Balb/c-mice sensitized by ovalbumin and children with IgE mediated atopic dermatitis. Randomized clinical trial were conducted, in 4 weeks probiotic administration. The adaptive immune response (IFN-h, IL-4 (Th2), and IL-10 (T reg), total IgE); and the allergic reaction were assessed. Both studies showed decreasing allergic reaction and increasing of IL-10 level. IgE level increased in animal model but showed no significant changes in human subjects and increased IL-4 and IFN-γ in animal models but decreased in human subjects. These findingsshowedthat Lactobacillus plantarum IS-10506 supplementation in animal model and human subjects were giving different immune responses, but both showed improvement in allergic reaction and increased T reg function which is represented by IL-10 levels. Lactobacillus plantarum IS-10506 offers a potential treatment for allergic diseases. Further long-term studies are required to confirm the therapeutic efficacy of LIS in allergic reaction.

    Time:

    Title: Using the Microbiome in Health and Disease : Perspectives and Challenges

    Francoise Le Vacon
    Biofortis Merieux Nutrisciences, France

    Biography
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    Biography

    Francoise Le Vacon
    Biofortis Merieux Nutrisciences, France

    Francoise is Chief Scientific Officer one of the founder of Biofortis Merieux NutriSciences. Francoise firstly obtained a Cellular and Molecular Biology Master from the University of Rennes, and then has been graduated from the Faculty of Sciences of Nantes in France. After different missions at Cochin Hospital and Pasteur Institute in Paris, Research Centre in Nantes, Johns Hopkins Hospital and the NIH in the US, she held research and development manager positions over the last 20 years. As Microbiome Research Program Leader, Francoise has set up a platform for the study of bacterial ecosystems for clinical and pre-clinical applications in nutrition, health and wellness. National Award : 2011 : Knight of the Order of Merit for contribution in Science.



    Abstract
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    Abstract

    Francoise Le Vacon
    Biofortis Merieux Nutrisciences, France

    Human health depends on a balance between three main components: Host Physiology and Psychology, Environmental Factors and Microbiome Ecosystem. From Pasteurian microbiology to the metagenomics revolution, the tremendous increase of scientific papers published on microbiome each year demonstrates that this field is one of the most promising of this century and offers us additional opportunities to advance in nutrition and medicine. Gut microbiome dysbiosis not only impact our Gut Intestinal (GI) metabolism but is also involved in numerous diseases in particular chronic illnesses, such as diabetes, IBD, cancer or neurologic diseases. In some conventional cancer therapy, new microbiome-adjuvant treatment could be prescribed. Next-generation probiotics candidates are known to be able to boost the response to immunotherapy treatment. Microbiome variations do not merely occur among individuals but can be temporal, spacial, health state and other intra-individual factor dependent. Today, the idea is to develop customized treatment strategies with microbiome intervention. With a personalised diet, precision prebiotics, probiotics or synbiotics tailored to individual patient characteristics, the goal is to re-equilibrate the microbiome. We face different challenges to improve our understanding of this complex ecosystem and to launch the novel microbial therapeutics or microbiome based- products.

    Time:

    Title: Altered gut microbiota in preterm newborns with necrotizing enterocolitis using High-Throughput sequencing

    Li Hong
    Shanghai JiaotongUniversity School of Medicine, China

    Biography
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    Biography

    Li Hong
    Shanghai JiaotongUniversity School of Medicine, China

    Dr. Li Hong is Professor of Pediatric Surgery at the Shanghai Childrens Medical Center, Shanghai Jiaotong University School of Medicine, and Director of the Department of Clinical Nutrition. Dr.Hong got her surgical training in Cincinnati Childrens Hospital and Chicago Comer Childrens Hospital, US, from 2007 - 2008. She also got her nutritional training as a clinical observer in Childrens Hospital of Los Angeles in 2010, and Boston Childrens Hospital from 2014 - 2015. She has played a major role in the development of Nutrition support strategies for Children in China. Her special interests include intestinal rehabilitation, and microbiome research in neonates.



    Abstract
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    Abstract

    Li Hong
    Shanghai JiaotongUniversity School of Medicine, China

    Recent studies have shown that pathophysiology of NEC includes intestinal microbial dysbiosis and mucosal barrier disruption. This study tends to investigate intestinal microbiota in preterm newborns with NEC. METHODS:- Our prospective study enrolled 24 preterm newborns admitted to the NICU in Shanghai Children’s Medical Center from March 2013 to August 2014, whose gestational age ranged from 29 to 33 weeks. Among the 24 preterm, 4 were diagnosed as NEC, while 3 were treated with antibiotics due to serious infections (infection group) and 17 without any infectious complications (normal group). Totally 192 longitudinal fecal samples were collected right from admission until discharge day. The Intestinal microbiota composition and its longitudinal trend were analyzed using Illumina-MiSeq high-throughput sequencing. RESULTS:- At phylum level, Firmutes and Proteobacteria dominated respectively in three groups, while Proteobacteria abundance of NEC group significantly ranked first (NEC vs Infection vs Normal: 59.84% vs 42.97% vs 44.13%, p=0.048). At class level, three groups shared the same domination microorganisms which are Bacilli, Clostridia and Gammaproteobacteria, and Gammaproteobacteriae abundance of NEC group is significantly the highest (NEC vs Infection vs Normal:53.63% vs 33.96% vs 39.46,p=0.018). Longitudinal comparison showed different microbial colonization pattern among three groups. Notwithstanding the same microbial development mode shared by three groups from Bacilli to Gammaproteobacteriawithin two weeks after birth, Bacilli and Clostridia domination from the 14th to 30th day of life and Clostridia domination after the 30th day of life in NEC group was distinctive and noticeable. CONCLUSIONS:- Abnormal intestinal microbiota at early life might account for NEC. However, more longitudinal studies with larger sample sizes are needed to revealmicrobial dysbiosis at different stages before and after the onset of NEC, hopefullyto provide evidence for its early recognition and prevention.

    Time:

    Title: Survival of encapsulated and free cells of probiotics in the gastrointestinal tract, using TIM-1 model

    Ingrid S Surono
    Bina Nusantara University, Indonesia

    Biography
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    Biography

    Ingrid S Surono
    Bina Nusantara University, Indonesia



    Abstract
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    Abstract

    Ingrid S Surono
    Bina Nusantara University, Indonesia

    Most of probiotics are members of the genera Lactobacillus and Bifidobacterium, which are inherently sensitive to gastric acid and bile. There is no generic probiotic, and effects are strain specific. A validated in vitro model of the GI tract (nick-named TIM) is able to predict survival of various strains in human clinical trials, and the model has been validated for survival of probiotics using volunteers that were intubated with a catheter until the end of the small intestine. Encapsulation by using alginatewas aimed to optimize the survival of strains. Encapulate and free cells of L. plantarum IS-10506 as well s E. faecium IS-27526 were assessed for the survial in GI tract by using TIM-1. The results show that the survival of encapsulate L.plantarum IS-10506 was much higher than the free cells. While there was no difference between encapsulated and free cells of strain E. faecium IS-27526. The survival of encapsulated and free cells of L.plantarum IS-10506 were 84.5% and 18.5%, respectively. L.plantarum survives 4.6 times better in encapsulated form than its free cells, when consumption of the viable counts are the same, and the survival rate of encapsulated was increasing after 2 hours of consumption, compared to the free cells. Taken together, encapsulation technique protects viability of cells to reach the GI tract.

    Time:

    Title: Oligosaccharides released from milk glycoproteins are selective growth substrates for infant-associated bifidobacteria

    Sercan Karav
    Canakkale Onsekiz Mart University, Turkey

    Biography
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    Biography

    Sercan Karav
    Canakkale Onsekiz Mart University, Turkey



    Abstract
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    Abstract

    Sercan Karav
    Canakkale Onsekiz Mart University, Turkey

    Milk, in addition to nourishing the neonate, provides a range of complex glycans whose construction ensures a specific enrichment of key members of the gut microbiota in the nursing infant, a consortium known as the milk-oriented microbiome. Milk glycoproteins are thought to function similarly, as specific growth substrates for bifidobacteria common to the breast fed infant gut. Recently, a cell wall-associated endo-β-N-acetylglucosaminidase (EndoBI-1) found in various infant-borne bifidobacteria was shown to remove a range of intact N-linked glycans. We hypothesized that these released oligosaccharide structures can serve as a sole source for the selective growth of bifidobacteria. Here, EndoBI-1 was used to release these N-glycans from concentrated bovine colostrum at the pilot scale. EndoBI-1-released N-glycans supported the rapid growth of Bifidobacterium longum subsp. infantis, a species that grows well on human milk oligosaccharides, but did not support growth of Bifidobacteriumanimalis subsp. lactis, a specieswhich does not. Conversely Bifidobacterium longum subsp. infantis ATCC 15697 did not grow on the deglycosylated milk protein fraction clearly demonstrating that the glycan portion of milk glycoproteins provides the key substrate for growth. Mass spectrometry-based profiling revealed that B.longum subsp. infantis consumed 73% of neutral and 92% of sialylated N-glycans, while B.animalis subsp. lactis only degraded 11% of neutral and virtually no (<1%) sialylated N-glycans. These results provide mechanistic support that N-linked glycoproteins from milk serve as selective substrates for the enrichment of infant-borne bifidobacteria capable of carrying out the initial deglycosylation. Moreover, released N-glycans are better growth substrates than the intact milk glycoproteins suggesting that EndoBI-1 cleavage is a key initial step in consumption of glycoproteins. Finally, the variety of N-glycans released from bovine milk glycoproteins suggests they may serve as novel prebiotic substrates with selective properties similar to those of human milk oligosaccharides.

  • Sessions:
    Probiotics in gastrointestinal interactions & disorders & Role of probiotics & prebiotics in human health & Probiotics in veterinary medicine

    Time:

    Title: Probiotics Reduce the Risk of Antibiotic-Associated Diarrhea in Adults (18 to 64 Years) but Not the Elderly (>65 Years): A Meta-Analysis

    Sadegh Jafarnejad
    Tehran University of Medical Sciences, Iran

    Biography
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    Biography

    Sadegh Jafarnejad
    Tehran University of Medical Sciences, Iran



    Abstract
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    Abstract

    Sadegh Jafarnejad
    Tehran University of Medical Sciences, Iran

    Antibiotic-associated diarrhea (AAD) is a common problem in adults and elderly patients due to the widespread use of antibiotics in this population. Multiple previous systematic reviews have demonstrated an association between specific probiotics and decrease of AAD, especially in children. As there is no specific analysis concerning the elderly patients, we decided to focus on adults, especially elderly people. Methods: We performed a systematic review of the literature regarding the use of probiotics in the treatment of AAD in adults (18–64 years old) and elderly subjects (≥65 years old). We identified 436 articles that met the search criteria. Thirty randomized controlled trials met the predefined inclusion criteria and were included in the meta-analysis. Results: There was considerable heterogeneity among the trials (P < .001); thus, subgroup analyses were performed. The meta-analysis resulted in a pooled relative risk (RR) of AAD of 0.69 (95% confidence interval [95% CI]: 0.62–0.76) in a fixed effects model and 0.58 (95% CI: 0.48–0.71) in a random effects model, as compared with placebo. The positive association between intake of probiotic and reduced risk of AAD was observed in adults (RR, 0.47; 95% CI: 0.4–0.56). In contrast, in elderly patients, there was no positive effect (RR, 0.94; 95% CI: 0.76–1.15) of probiotic use and AAD. Conclusion: In summary, the results emerging from our meta-analysis suggested that adjunct probiotic administration is associated with a reduced risk of AAD in adults but not in elderly people.

    Time:

    Title: Eubiotics: Future in Biotechnology Industry

    Naheed Mojgani
    Razi vaccine and Serum Research Institute, Iran

    Biography
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    Biography

    Naheed Mojgani
    Razi vaccine and Serum Research Institute, Iran



    Abstract
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    Abstract

    Naheed Mojgani
    Razi vaccine and Serum Research Institute, Iran

    With the ban on the use of antibiotics as growth promoters and increasing consumer awareness with respect to preventive health care and nutrition, high demand for safe human food has fueled eubiotic market. The term Eubiotics is mainly used in the feed industry where it refers to a healthy balance of the micro-flora in the gastrointestinal tract. Bacteritic preparations intended for correction of biocenosis of the mucous membranes may be referred to as eubiotics. Poultry feed dominates the eubiotic market owing mainly to the large number of population consuming poultry meat in the world. The demand for eubiotics in animal feed is projected to grow during the forecast period, owing to the rapidly increasing global demand for quality animal products. Probiotics, prebiotics, essential oils and organic acids are often referred to as eubiotics. In this context, Probiotics account for the major share in poultry feed. It is hypothesized that different eubiotic combinations would have a synergistic effect in improving animal performance. In Iran, in last decade a number of local probiotic manufacturers have evolved which are producing high quality probiotic feed additives for animals. While, this trend is still in its developing and pillaring stages, a number of researches on eubiotic products with medical and animal applications are also on its rise. According to global estimates, Europe dominates the eubiotics market followed by North America, while, China, India, and Brazil are the developing countries where eubiotic market is also growing. The biotechnology industry is now creating an opportunity for growth in the eubiotics sector. A number of eubiotic drugs are being developed especially for treating dysbiosis. However, factors such as low shelf life of eubiotics and high cost of eubiotics leading to the rise in cost of production are restraining the growth of the market. With high tech approaches and biotechnological innovations we foresee a bright future for eubiotic products in Iran. Hopefully with entering the new era of Biotechnology we will soon witness highlight eubiotic products in the country with wide applications.

    Time:

    Title: Screening of purine nucleosides degrading probiotic lactic acid bacteria as a novel approach in management of hyperuricemia

    Neha Pandey
    National Dairy Research Institute, India

    Biography
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    Biography

    Neha Pandey
    National Dairy Research Institute, India



    Abstract
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    Abstract

    Neha Pandey
    National Dairy Research Institute, India

    Out of 20 different curd and fecal samples, 76 Gram positive and catalase negative rods were isolated and screened for purine nucleoside degradation ability. A HPLC device equipped with a Photo diode array (PDA) detector was used to evaluate the ability of these isolates to degrade inosine and guanosine, the two key intermediates in purine metabolism by incubating these isolates with inosine-guanosine solution (1.25mM) for 2 h at 37oC. Contents of remaining inosine and guanosine were identified at 254 nm by retention time of 7.48 and 6.46 min, respectively, and quantified by interpolation of calibration curves. To evaluate the degradation of purine compounds by cell-free extracts of LAB, the cells were sonicated for 30’X3 (pulse rate- 5sec on/ 5 sec off) and activity evaluated through HPLC. Eleven isolates viz. D3A, D1AB, D1F, D3E, D6F, D2A, D5F, D3D, D3B, D1AC, D1AA were screened as positive for purine nucleoside degradation ability. These organisms were then evaluated for their potential probiotic attributes. Out of these 11 isolates only six isolates viz. D3A, D1AB, D2A, D6F, D5F and D1AA were found to be most acid tolerant and selected for further in vitro evaluation. When tested for bile tolerance only three isolates viz. D6f, D5F, D1AA and D3A were found to resist 2% bile and showed highest viability among the tested six strains. Further evaluation of the isolate for antimicrobial activity showed that the all the screened four isolates showed a good antimicrobial activity against Gram positive bacteria viz. Enterococcus faecalis, Bacillus subtilis, Staphylococcus aureus, Microccusluteus and other Lactobacillus species. Our work shows that our isolates apart from purine degradation are good probiotics as proved by their acid and bile tolerance. Also, the antimicrobial activity showed by the isolate can give an added advantage to the isolate to compete and survive in the GI tract.

    Time:

    Title: The effects of a probiotic protocol on salivary biomarkers, heart rate variability, and daily wellness scores in elite Australian athletes - Results of a double-blind randomised controlled trial

    Joanna Harnett
    The University of Sydney, Australia

    Biography
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    Biography

    Joanna Harnett
    The University of Sydney, Australia

    Dr Joanna Harnett holds a PhD in nutritional pharmacology and a bachelor and master degree in health science. She currently holds a fulltime teaching and research position within the Faculty of Pharmacy at The University of Sydney Australia. Her PhD (2013) explored the association between the intestinal microbiota and coeliac disease and included a randomized controlled trial evaluating the effects of a probiotic formulation on the composition of the faecal microbiota and symptom scores. Her current research activities include collaborating with Associate Professors Kate Pumpa (Sports Dietician) and Andrew McKune (Exercise Physiologist) exploring the role of probiotics in elite athletes.



    Abstract
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    Abstract

    Joanna Harnett
    The University of Sydney, Australia

    Objective: To evaluate the effects of a probiotic protocol formulation on the salivary biomarkers sIgA, cortisol, alpha-amylase and psychometric measurements in elite athletes. Methods:A double-blindrandomised controlled trial was conducted over 17 weeks. Elite male Australian Rugby Union athletes were randomly assigned to receive either aprobiotic (n=11) or a placebo (n=10) supplement. The probiotic Ultrabiotic 60(TM) or placebo was taken with food twice daily for 13weeks and SB Floractiv(TM) 250mg twice daily during athletes international travel period (9 weeks). The main outcome measured included salivary alpha-amylasesAA, cortisol, testosterone and secretory immunoglobulin A (sIgA), heart rate variability (HRV) and daily wellness scores including sleep quality, motivation and muscle soreness. Results: There were no significant differences between the groups at baseline: age p=0.77, and weight (p=0.96). Multiple linear regression analysis identified alpha amylase and LF/HF ratio (HRV) were both independent predictors of general muscle pain and together account for 15.31% of the variance in general muscle pain in the placebo group (p < 0.0001). Sleep quality and motivation were both independent predictors of reducedmuscle pain and together account for 40.43% of the variance in general muscle pain (p < 0.0001). HRV and alpha-amylase were weakly associated with general muscle pain in the probiotic group. Conclusion: The probiotic protocol trialed in this study resulted in a positive effect on sleep quality and motivation scores that is associated with a reduction in perceived muscle pain. Further research is required to evaluate this association.

    Time:

    Title: Impact of Supplementation of the Sudanese Fermented Milk (Robe) Supplemented with the Gum Arabic Powder

    Abdel Moneim Sulieman
    University of Hail, Kingdom of Saudi Arabia

    Biography
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    Biography

    Abdel Moneim Sulieman
    University of Hail, Kingdom of Saudi Arabia

    Prof. Abdel Moneim has been awarded his PhD in 2001 and pursued a postdoctoral fellowship at the University of Kobe, Japan. He is expert in “Food Science and Technology, his main concern is food microbiology. Prof. Abdel Moneim has authored a large number of articles in reputed journals and has been invited to different international conferences. He published many books in the area of food science and technology. He is a member of many national and international academic associations.



    Abstract
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    Abstract

    Abdel Moneim Sulieman
    University of Hail, Kingdom of Saudi Arabia

    Gum Arabic is a natural prebiotic non-digestible polysaccharides known with its ability to regulate bowel movement and assists digestion. Consumption of fermented sour milk (Robe) is part of Sudanese culture. It is the main traditionally fermented milk product of the Sudan. In this study four types of Robe samples were prepared: control Robe was prepared using previous batch of Rob (2.5%), gum Robe using three levels of gum powder 5%, 7.5% and 10%. The effect of addition of gum on chemical characteristics of rob was investigated. The chemical analysis showed that the protein content of control sample (2.37% ) was higher than That of gum rob (1.78) for 10% gum rob. The total soluble solids (TSS) highly increased from 8.92% in the control sample to 16.41% in 10% gum rob sample. Moreover, the addition of gum powder resulted in decreasing the moisture content from 91.69% (control sample) to 83.76% (10% gum rob), this may be due to gum which has high water holding capacity which enhances the texture of rob. The result also indicated that the ash content of gum rob (0.91%) was relatively higher than that of the control rob (0.66%). On the other hand, the whey separation decreased with the addition of gum (5.67 ml for control) – (1.55 ml for 10% gum rob). Statistical analysis indicate that there were no significant differences in pH, Acidity, Lactose and fat between the samples. The sensory evaluation indicate acceptance of rob prepared by adding gum powder due to the improvement of rob texture .

    Time:

    Title: In vitro probiotic characteristics, antioxidant, anticancer properties of selected lactic acid bacteria strains

    Mohamed Shehata
    Universities and Research Centers District Borg El-Arab City, Alexandria, Egypt

    Biography
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    Biography

    Mohamed Shehata
    Universities and Research Centers District Borg El-Arab City, Alexandria, Egypt



    Abstract
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    Abstract

    Mohamed Shehata
    Universities and Research Centers District Borg El-Arab City, Alexandria, Egypt

    Probiotic strains can probably be applied as bio-preservatives and functional food supplement. Eight lactic acid bacteria strains (LAB) Lactobacillus brevis NRRL B-4527; Streptococcus Thermophilus BLM 58;Pediococcusacidilactici ATCC 8042; Lactobacillus rhamnosus CCUG 1452; Lactobacillus curvatus ATCC 51436; Lactococcuslactis sub sp. lactisDSM 20481; Lactobacillus plantarum DMSZ 20079 and Lactobacillus plantarumTF103 were selected to screen the probiotic properties, antioxidant and anticancer potential. LAB strains exhibited showed antimicrobial activity against food-borne pathogenic. Further, in vitro probiotic properties of eight strains displayed excellent acid tolerance, bile tolerance, simulated gastrointestinal juice tolerance, in vitro adhesion ability for Caco-2 cell line. The antioxidant effect of intracellular and cell-free extract of lactic acid bacteria strains was evaluated by various antioxidant assays, namely, resistance to hydrogen peroxide, DPPH radical scavenging, ABTS radical scavenging, and hydroxyl radical scavenging (HRS). The results showed that intracellular and cell-free supernatant of S. Thermophilus BLM 58, L. lactissubsp.lactis DSM 20481, P. acidilactici ATCC 8042, L. brevis NRRL B-4527strains possess excellent antioxidant capacity. The intracellular of S. Thermophilus BLM 58 and P. acidilactici ATCC 8042 also showed the highest anticancer activity against Caco-2, MCF-7, HepG-2 and PC-3cells. Antioxidant, antimicrobial and anticancer characteristic of elected probiotic S. Thermophilus BLM 58 and P. acidilactici ATCC 8042 would be valuable in the functional food production industry and therapeutic formulations.

    Time:

    Title: Probiotics in celiac disease: Simulated gut hydrolysis of gluten

    Maria De Angelis
    University of Bari Aldo Moro, Italy

    Biography
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    Biography

    Maria De Angelis
    University of Bari Aldo Moro, Italy



    Abstract
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    Abstract

    Maria De Angelis
    University of Bari Aldo Moro, Italy

    The aim of this study was to demonstrate the capacity of probiotic lactobacilli to hydrolyze immunogenic gluten peptides. Eighteen commercial strains of probiotic lactobacilli with highly variable peptidase activity (i.e., aminopeptidase N, iminopeptidase, prolylendopeptidyl peptidase, tripeptidase, prolidase, prolinase and dipeptidase), including towards Pro-rich peptides, were tested in this study. Ten probiotic strains were selected based on their specific enzyme activity. When pooled, these ten strains provide the peptidase portfolio that is required to completely degrade the immunogenic gluten peptides involved in celiac disease (CD). The selected probiotic mixture was able to completely hydrolyze well-known immunogenic epitopes, including the gliadin 33-mer, α9-gliadin peptide 57-68, A-gliadin peptide 62-75, and γ-gliadin peptide 62-75. During digestion under simulated gastrointestinal conditions, the pool of 10 selected probiotic lactobacilli strongly hydrolyzed the wheat bread gluten to less than 10 ppm after 360 min of treatment. As determined by multidimensional chromatography (MDLC) coupled to nanoelectrospray (ESI)-mass spectrometry (MS/MS), no known immunogenic peptides were detected in wheat bread that was digested in the presence of the probiotics. Accordingly, the level of cytokines (IL-2, IL-10 and IFN-γ) produced by duodenal biopsies of CD patients who consumed wheat bread digested by probiotics was similar to the baseline value (negative control). Probiotics that specifically hydrolyze gluten polypeptides could also be used to hydrolyze immunogenic peptides that contaminate gluten-free products. This could provide a new and safe adjunctive therapy alternative to the gluten-free diet.

    Time:

    Title: The effect of microencapsulation with genipin cross-linked chitosan and poly L-lysine coatings and different starches on survival of probiotics in simulated gastro-intestinal condition, freezing and heat treatment

    Mohammad Ali Khosravi Zanjani
    Islamic Azad University, Iran

    Biography
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    Biography

    Mohammad Ali Khosravi Zanjani
    Islamic Azad University, Iran



    Abstract
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    Abstract

    Mohammad Ali Khosravi Zanjani
    Islamic Azad University, Iran

    In this study, microencapsulation of the probiotics Lactobacillus rhamnosus (ATCC 7469) and Bifidobacterium bifidum (ATCC 29521) was carried out using calcium alginate, potato, rice and high-amylose corn (Hylon VII) starches along with genipin cross-linked chitosan and poly-L-lysine coatings. The viability of probiotics was investigated under heat treatment (72, 85, and 90 deg.C, 0.5 min), freezing condition (-30deg.C, 100 days), simulated gastric juice (along with pepsin, pH = 2, 2 h at 37 C), and simulated intestinal juice (along with pancreatin and bile salts, pH=8, 2 h at 37 C). The morphology and size of microcapsules were measured by scanning electron microscopy and particle size analyzer. Results of this research demonstrated that, compared with the free form, microencapsulated probiotics had significantly (P < 0.05) higher viability under simulated gastro-intestinal conditions, freezing condition and heat treatment. Coating the capsules with chitosan and poly L-lysine led to enhanced the viability of bacteria and an increase in size of microcapsules. Microcapsules with genipin cross-linking significantly increased the viability of probiotics compared with non-cross-linked microcapsules. Moreover, genipin did not influence the size of the microcapsules produced using the emulsion technique. In general, this research indicated that the presence of genipin as a cross-linking agent in the structure of coatings considerably enhanced the viability of encapsulated probiotics due to modification of the physical structure. Among different starches, the presence of Hylon (high-amylose starch) as a material resistant to heat, frozen storage and digestive enzymes, not only increased the viability of probiotics in simulated human gastro-intestinal condition but also considerably improved the thermal and freezing resistance of microcapsules.

    Time:

    Title: The role of probiotics in the poultry industry

    Ozlem Osmanagaoglu
    Ankara University, Turkey

    Biography
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    Biography

    Ozlem Osmanagaoglu
    Ankara University, Turkey

    Ozlem Osmanagaoglu graduated from the Biology Department of Middle East Technical University in Turkey. Later, she completed her master and PhD program on genetics of Lactic Acid Bacteria at the Biotechnology Institute of METU. During her doctoral and post doctoral periods, she worked under NATO grant and scholarship in the University of Wyoming, USA. Presently, she is a member of the Biology Department within the Science Faculty of Ankara University in Turkey where she has established Microbial Genetics Laboratory with a young productive research team. Her work has focused on bacteriocins, natural antimicrobial peptides, produced by Lactic Acid Bacteria, their molecular typing and probiotics.



    Abstract
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    Abstract

    Ozlem Osmanagaoglu
    Ankara University, Turkey

    For more than 50 years, antibiotics have been used in poultry industry extensively both as growth promoters and therapeutic agents to prevent or control poultry diseases, to promote growth and feed efficiency, to enhance poultry production as well as to treat sick animals before they were banned in many countries due to the fear of resistance development in bacterial populations to antibiotics in exposed individuals, presence of antibiotic residues in poultry products intended for human consumption such as meat and eggs and the increasing emergent consumer demand for products free from antibiotic residues. With increasing concern about antibiotic resistance and the ban on sub-therapeutic use of antibiotics in Europe and the US, it has become important in poultry production to find alternative products that could replace antibiotics, without any loss of productivity/product quality or negative influence on health. An alternative approach is the use of probiotics which can be defined as ‘‘live microorganisms which, when administered in adequate amounts, confer a health benefit on the host by improving its intestinal balance’’. Among probiotics used in poultry production, although lactic acid bacteria (LAB) such as Bifidobacterium, Lactobacillus, Lactococcus, Enterococcus and Pediococcus species are the ones most commonly used; Bacillus, E. coli, Streptococcus species, a range of yeast species such as Saccharomyces and non-defined mixed cultures have also been used. As compared to antibiotics, probiotics do not leave residues in the poultry products, which may have serious health implications for consumers. Besides, the addition of probiotics provides competition to pathogenic organism for intestinal colonizing sites, replaces enteric pathogens by means of competitive exclusion in the poultry intestinal tract, thereby increase the intestinal health of poultry by reducing pathogenic infections and protect the host against pathogens, which is relevant in promoting animal growth as well as improving the productivity (performance of poultry) and immunity of the host.

    Time:

    Title: Use of probiotics to control gastrointestinal nematodes in sheep under grazing conditions, in Uruguay

    America Mederos
    Instituto Nacional de Investigacion Agropecuaria, Uruguay

    Biography
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    Biography

    America Mederos
    Instituto Nacional de Investigacion Agropecuaria, Uruguay

    América Mederos has a Veterinary Medicine degree from the Veterinary School in Montevideo, Uruguay. During 1993 she completed her MSs at Reading University (UK) and his PhD at the University of Guelph in 2010 in Veterinary Epidemiology. Since 1991 she joined the National Research Institute for Agriculture and has been conducting research programs on Animal Health. Her main interest has been in the study of anthelmintic resistance and searching for alternative methods to control gastrointestinal nematodes in ruminants using non-chemical drugs such as bioactive forages and probiotics.



    Abstract
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    Abstract

    America Mederos
    Instituto Nacional de Investigacion Agropecuaria, Uruguay

    In Uruguay, anthelmintic resistance is a wide-spread phenomenon among sheep farms and there is a need to search for alternative strategies. The aim of this study was to evaluate non-chemical alternatives to control GIN in sheep under grazing conditions in Uruguay. Trial1 was conducted at the Research Station “La Estanzuela”, Colonia using 110 lambs distributed in five groups (n=22) with two repetitions each: G1= feed 16% protein concentrate (Control); G2=16% protein concentrate + 1% condensed tannins; G3= % protein concentrate + 4% condensed tannins; G4= 16% protein concentrate + condensed tannins extract as oral drench; G5= 16% protein concentrate + Saccharomyces cerevisiae CNCM I-1077 (LEVUCELL®SC).Trial2 was conducted at a commercial farm in Artigas using 90 lambs. Three experimental groups (n=30) were formed grazing native grasses: G1= feed 20% protein concentrate + Saccharomyces cerevisiae CNCM I-1077 (LEVUCELL®SC); G2= feed 20% protein concentrate (Control) and G3= feed 20% protein concentrate + 1% condensed tannins. Trial3 was similar to Trial 2 conducted during 2016. The follow up period for Trial1 and Trial2 was from January- June 2015 and for Trial3 from January-June 2016. Production traits were recorded and fecal egg counts (FECs) were performed on individual animals. Infective larvae were recorded in animals and pasture. Briefly, the main effect of probiotic treatment wasa significant reduction in the number of treatments the animals required to thrive in Trial2 and Trial3 (31 vs 44 and 11 vs 30 respectively), in comparison with the control (p<0.05). In Trial1, FECs tended to be lower in G5 compared with G1 (2448 vs 2898) and G1 compared with G2in Trial2 (900 vs 1100). Although probiotics have proven to be effective in controlling microbes, the results obtained from these exploratory trials are promising for further studies.

    Sessions:
    Probiotics in womens health & Prospects of probiotics vision, opportunity and challenges & imunne support by probiotics & Recombinant Probiotics

    Time:

    Title: Effects of a Multi-species Probiotic Supplement on Bone Health in Osteopenic Postmenopausal Women: a Randomized, Double-blind, Controlled Trial

    Sadegh Jafarnejad
    Tehran University of Medical Sciences, Iran

    Biography
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    Biography

    Sadegh Jafarnejad
    Tehran University of Medical Sciences, Iran



    Abstract
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    Abstract

    Sadegh Jafarnejad
    Tehran University of Medical Sciences, Iran

    The development of alternative approaches to prevent and/or treat osteoporosis, as a chronic progressive bone disease, is being considered currently. Among the dietary supplements, probiotics may have favorable effects on bone metabolism. Therefore, the aim of this study was to evaluate the effects of a multi-species probiotic supplementation on bone biomarkers and bone density in osteopenic postmenopausal women. Methods: This randomized double-blind placebocontrolled clinical trial was performed on 50 osteopenic patients aged 50–72 years. Participants were randomly assigned to take either a multi-species probiotic supplement (GeriLact) (n = 25) or placebo (n = 25) for six months. GeriLact contains seven probiotic bacteria species. Participants received 500 mg Ca plus 200 IU vitamin D daily. Bone mineral density (BMD) of lumbar spine and total hip, blood biomarkers including bone-specific alkaline phosphatase (BALP), osteocalcin (OC), collagen type 1 cross-linked C-telopeptide (CTX), deoxypyridinoline (DPD), parathyroid hormone (PTH), 25-OH vitamin D, and serum pro-inflammatory cytokines (TNFα and IL-1β) were assessed at baseline and at the end of the study. Results: The multispecies probiotic significantly decreased BALP (P=0.03) and CTX (P=0.04) levels in comparison with the control group, but had no effect on BMD of the spine and total hip. Moreover, there was a statistically significant decrease in serum PTH (P=0.01) and TNFα (P=0.02) in the intervention group compared with placebo group. Conclusions: These results may suggest the favorable effects of the multi-species probiotic supplementation for six months on bone health in postmenopausal women due to slowing down the rate of bone turnover.

    Time:

    Title: Probiotics, an opportunity for mental and metabolic health

    Nina Vinot
    Probiotical, Italy

    Biography
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    Biography

    Nina Vinot
    Probiotical, Italy

    Before entering industry, Nina was involved in nutritional research at Penn State University, USA, the French National Centre for Scientific Research and the National Institute for Agronomic Research. Today she manages sales across Western Europe markets for Probiotical. She has a degree in Agronomy and Nutrition from AgroParisTech, National Institute of Life Sciences



    Abstract
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    Abstract

    Nina Vinot
    Probiotical, Italy

    From an overview on the increasing science publications relative to probiotics and the microbiota, as well as an increasing global market for probiotics, we will discuss how the reach of probiotics is more and more known and acknowledged in always more aspects of our health. The biggest interest seen currently is orientated towards the gut brain axis and how probiotics can help people with depression and anxiety disorders, mood, autism or even Parkinson disease. We will explain some of the pathways with which the gut communicates with the brain and vice-versa, and the potential of probiotics. Secondly, given the present epidemic of obesity and metabolic diseases and the increasingly established role of the microbiota in these diseases, we will discuss on the opportunities of probiotics and fecal transplant regarding weight management, cholesterol control, the TMAO pathway and its role in atherosclerosis, as well as diabetes. Probiotics and microbiota restauration hold a special role as they have been shown to help people while re-establishing a natural balance, in all safety and without side effects, bringing potential alternatives to certain drugs. The opportunities brought by public awareness, scientific evidence and demand from the market are limited by some challenges faced with the industry, regarding the technical feasibility of new strains, the regulatory hurdles around claiming and customer expectations of pharma quality and studies while the products are mostly staying under the status of food or food supplement with a limited cost per dose to the consumer.

    Time:

    Title: Probiotics and/in P4: personalization, prevention, prediction and patients

    Nadiya Boyko
    Uzhhorod National University & Cassovia Life Science, Ukraine

    Biography
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    Biography

    Nadiya Boyko
    Uzhhorod National University & Cassovia Life Science, Ukraine

    Nadiya Boyko, PhD, DSc. in Microbiology, 5 years sabbatical Research Fellow in Laboratory of Mucosal Immunology, University of Pennsylvania, USA, now Professor at the Faculty of Medicine of Uzhhorod National University, Director of the R&D Centre of Molecular Microbiology and Mucosal Immunology. She has published more than 200 papers, including chapter in Mucosal immunology Elsevier press. She is an expert is pharmabiotics and personalised nutrition, regulation of human [gut] microbiota for prevention of noncommunicable diseases. She is member of SMI, SOMED, ASA, SMU. International project: FP7 (BaSeFood, JSO-ERA), CEE: CAPINFOOD, FoodWARD (Erasmus+), COST: BacFoodNet, ODiNand SKIN



    Abstract
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    Abstract

    Nadiya Boyko
    Uzhhorod National University & Cassovia Life Science, Ukraine

    New ideas about the uniqueness of the human microbiome and its decisive role in the health of the population today substantiate the idea of individualization of treatment approaches for patients (patient stratification) and individualized prevention. The latter being the prevention of diseases of the population through the specific implementation of personalized nutrition. Embodied in this is the selection of different biotics (pro-, pre-, syn-, pharma- and immuno-biotics).They need to be 1) unique to each individual; 2) specific, and therefore diagnostic for each type of disease. Based on these approaches we created the new human pharmabiotics. At the core of both biotics modifiers is the initial composition of synergistically selected microorganisms of the normal commensal microbiota and prebiotic ingredients prescreened and extracted from edible plants. Their composition can be changed and adapted according to the individual patients’ requirements and to any nosology. This is done by selecting the necessary components and their specific combination in accordance with existing bioactive substances databases and their impact on representatives of commensal, pathogenic and opportunistically pathogenic microbiota. We had tested their ability specifically modulate local and systemic immune response in experiments in numerous animal models and also on human dendritic cells derived from peripheral blood monocytes. One of the key aspects in the development of such specified, truly personalized bioticsis the geographical aspect, because on the one hand the microbiome state is adjusted by different local epigenetic factors including diet (composition, properties, content of biologically active compounds and impact) and on the other hand, determined by local factors. In light of the aforementioned, we believe that such biotics are as promising as mono- or fixed structure pro-, pre- and synbiotics due to the fact that in addition to the impact of functionality regulation, their selectivity also helps to restore the individuals’ microbiome biodiversity.

    Time:

    Title: Bacillus probiotics as antimicrobials

    Iryna Sorokulova
    Auburn University, USA

    Biography
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    Biography

    Iryna Sorokulova
    Auburn University, USA

    Dr. Iryna Sorokulova, Professor of Microbiology, Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, AL, USA. She received her MS degree in Microbiology from Taras Shevchenko Kiev State University (Ukraine) and Ph.D. and D.Sc. Degrees in Microbiology from Institute of Microbiology and Virology, National Academy of Sciences of Ukraine. Dr. Sorokulova is the author of 92 refereed scientific publications, 4 books, and 23 patents (1- US, 1-France; 6-Ukraine, 15-Russia). Six patents are now licensed and commercialized. She created several biotechnological products; some of them are commercially available.



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    Abstract

    Iryna Sorokulova
    Auburn University, USA

    Antibiotic resistance in pathogens was identified as a serious health threat, which is associated with increased morbidity and mortality worldwide. The total economic cost of antibiotic resistance to the US and EU healthcare systems was calculated as $20-35 and EUR 1.5 billion a year correspondingly. The emergence of multiresistant pathogens requires the development of new approaches to their control. Probiotic prophylaxes and therapies are gaining wider acceptance as more scientific data emerge regarding the interaction between pathogen and beneficial microbes in the human intestinal tract and molecular mechanisms of probiotics’ action. Probiotic bacteria which confer beneficial effect for the host and have pronounced antagonistic activity against pathogens is expected to present a clear alternative for control of drug-resistant infections. Bacteria of the Bacillus genus are known as potent producers of a wide variety of antimicrobial compounds. These bacteria are also reputed to promote health benefits on the host. We believe that Bacillus bacteria with high activity against pathogens can be a valuable alternative strategy to control multi-resistant infections. Our study showed high activity of B. subtilisprobiotic strain against a broad spectrum of pathogens, including multiresistant S. aureus, Salmonella, Candida. We also found an inhibitory effect of this strain on influenza virus in vitro and in vivo studies. New peptide P18, produced by B. subtilis strain was isolated and characterized. Peptide P18 was not toxic and completely inhibited influenza virus at concentration 12.5 µg/mL. In animal studies theantiviral effect of P18 was comparable with Tamiflu. In conclusion, our results showed that Bacillus probiotic can be used as a valuable new approach for treatment of bacterial and viral infections.

    Time:

    Title: Effect of maize based corn fibre combined with Lactobacillus rhamnosus LGG and the pilus-­deficient derivative GG-­PB12 on faecal microbiota, immune function and metabolism in healthy elderly (Saimes study)

    Adele Costabile
    University of Roehampton, London

    Biography
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    Biography

    Adele Costabile
    University of Roehampton, London

    Dr Costabile joined the University of Roehampton, Health Sciences Research Centre in August 2015 as a Lecturer in Nutrition. Dr Costabile research interest and publications focus on the interactions between human nutrition and gut microbiology. Her publication record has been built through ten years of research fellowship in Professor Glenn Gibson group at the University of Reading where she has lead several humanfeeding studies, often built on data from initial in vitro microbiology experiments which have resulted in a number of functional food products becoming established within the European market. Dr Costabile research outcomes have broadened our understanding of the interaction between gut microbiome and the host in both health and disease, as well as the modulation of gut microbiome through functional foods. Specifically, Dr Costabile has formed strategic alliances with leading researchers in the fields of human nutrition, metabonomics, lipidomics, immunology and food! Bio-­‐processing. These collaborations have led to joint publications, grant applications and financial! support.



    Abstract
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    Abstract

    Adele Costabile
    University of Roehampton, London

    The aging process leads to a potential decline in immune function and adversely affects the gut microbiota. Dietary intervention is one approach to affect gut microbiota composition and improved functioning of the immune system. We investigated the effects of a probiotic Lactobacillus rhamnosus GG (LGG) and pilus-­‐ deficient L. rhamnosus GG-­‐PB12 combined with PromitorTM Soluble Corn Fibre (SCF, a candidate prebiotic) on fecal microbiota, metabolism, immunity and blood lipids in healthy elderly persons. A prospective, double-­‐blind, placebo controlled, randomized, single centered, cross-­‐over study in 40 healthy elderly subjects (aged 60-­‐80y) was carried out. Volunteers were randomized to consume either probiotic and prebiotic as synbiotic, prebiotic or placebo (maltodextrin) during 3-­‐weeks. Three-­‐week wash-­‐out periods separated all the treatments. We assessed effects upon blood lipids, glucose, cytokines, NK cell activity, phenotype and intestinal microbiota composition. SCF decreased IL-­‐6, which was not observed with the synbiotics. Consumption of L. rhamnosus GG combined with SCF increased NK cell activity compared to baseline in females and the older group. In the fecal microbiota analyses, the strongest community shifts were due to L. rhamnosus GG combined with SCF and SCF treatments. L. rhamnosus GG combined with SCF and L. rhamnosus GG-­‐PB12 combined with SCF significantly increased the genus Parabacteroides. L. rhamnosus GG combined with SCF and SCF increased concentrations of Ruminococcaceae Incertae sedis. Oscillospira and Desulfovibrio slightly decreased in the L. rhamnosus GG combined with SCF group, whereas Desulfovibrio decreased also in the L. rhamnosus GG-­‐PB12 combined with SCF group. L.rhamnosus GG combined with SCF reduced total cholesterol and LDL-­‐cholesterol in volunteers with initially elevated concentrations. CRP decreased during L. rhamnosus GG-­‐ PB12 combined with SCF intervention compared to baseline. Conclusions: Piliated L. rhamnosus GG was more potent at inducing beneficial effects compared to L. rhamnosus GG-­‐PB12 by modulating the microbiome, increasing NK cell activity compared to SCF alone in older volunteers and decrease of the pro-­‐inflammatory cytokine IL-­‐6.

    Time:

    Title: Developing a vaccine against the porcine rotavirus using Lactobacillus plantaram cells

    Seria Shonyela
    Jilin Agricultural University, China

    Biography
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    Biography

    Seria Shonyela
    Jilin Agricultural University, China



    Abstract
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    Abstract

    Seria Shonyela
    Jilin Agricultural University, China

    Porcine rotavirus infection is a significant cause of morbidity and mortality in the pig industry necessitating the development of effective vaccines for the prevention of infection. Gut mucosal immune responses are likely to play significant role in protective immunity against rotavirus infection because rotaviruses are enteric pathogens. Lactic acid bacteria (LAB) are Gram-positive, nonpathogenic microorganisms that are gaining to a great concern as antigen producers for improvement of live vaccine vectors. Heterologous proteins of dissimilar source have been effectively expressed in various LAB species. Recombinant L. plantaram NC8 strains have been shown to induce specific local and systemic immune responses against a range of antigens. The objective of this study was constructing a Lactobacillus plantaram NC8 strain expressing the heterologous VP7 porcine rotavirus protein and investigating its outcome and its ability acting as an antigen delivery system for oral vaccinations on mice. The expression of recombinant pSIP409-VP7-DCpep was confirmed by SDS-PAGE and Western blot analysis and surface-displayed expression on L. plantarum was verified by immunofluorescence. Mice orally immunized with recombinant protein-expressing L. plantarum produced high levels of serum immunoglobulin G (IgG) and mucosal IgA. The IgA titters from mice immunized with NC8-pSIP409-VP7-DCpep were higher than titters from pSIP409-VP7-DCpep -immunized mice. The induced antibodies demonstrated neutralizing effects on RV infection. The outcome of this study is a hopeful step toward developing a vaccine against the porcine rotavirus using Lactobacillus plantaram cells as bioreactors for competent antigen production and delivery to the mucosal surface.

  • Sessions:
    Poster Presentation

    Time:

    Title: Indigenous Probiotic LactobacillusIsolates Presenting Antibiotic like Activity against Human Pathogenic Bacteria

    Debashis Halder
    University of Gour Banga, India

    Biography
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    Biography

    Debashis Halder
    University of Gour Banga, India

    Debashis Halder is involved in Ph. D Research since last three years, under the supervision of Prof. Shyamapada Mandal, Laboratory of Microbiology and Experimental Medicine, Department of Zoology, University of GourBanga, India.He has worked on three projects and published five research articles in four reputed international journals. He is passionate to pursue a career in Medical Microbiology and truly attracted by Molecular Immunology and Phytomedicine. Hewishes to continue as a scientist with a dynamic team of sincere researchers along with continuous research in his fields and extend his valuable service towards the scientific community with extensive research work.



    Abstract
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    Abstract

    Debashis Halder
    University of Gour Banga, India

    Indigenous lactic acid bacteria are well known probiotics having antibacterialactivity against potentially pathogenic bacteria. This study aims to characterize the curd lactobacilli for their probiotic potentiality and antagonistic activity against clinical bacteria. Methods: Four curd samples were processed microbiologically for the isolation of lactic acid bacteria (LAB). The LAB strains obtained were identified by conventional methods: cultural aspect, gram-staining, biochemical and sugar fermentation tests. The probiotic properties were justified with tolerance to low-pH, bile salt and sodium chloride, and the antagonistic activity of the lactobacilli against human pathogenic bacteria (Escherichia coli, Proteus vulgaris, Acinetobacterbaumanniiand Salmonella entericaserovarTyphi) was assessed. Hemolytic activity and antibiotic susceptibility were determined for the lactobacilli isolates, and the cumulative probiotic potential (CPP) values were recorded. Result: Four lactobacilli isolates, L. animalisLMEM6, L. plantarumLMEM7, L. acidophilusLMEM8 and L. rhamnosusLMEM9, procured from the curd samples, survived in low-pH and high bile salt conditions, and showed growth inhibitory activity against the indicator bacteria by agar-well (zone diameter of inhibition; ZDIs: 13.67 ±0.58–29.50 ±2.10 mm) and agar overlay (ZDIs: 11.33 ±0.58–35.67 ±2.52 mm) methods; the average growth inhibitory activity of lactobacilli ranged 233.34 ±45.54–280.56 ±83.67 AU/mL, against the test bacterial pathogens. All the lactobacilli were non-hemolytic and sensitive to most of the test antibiotics. The CPP values of the isolated LAB were recorded as 80–100%. Conclusion: The curd lactobacilli procured might be used as the valid candidates of probiotics, and bio-therapeutics against bacterial infection to humans.

    Time:

    Title: Encapsulation of Pediococcuspentosaceus OZF, a promising probiotic bacterium

    Harun Onlu
    Muş Alparslan University, Turkey

    Biography
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    Biography

    Harun Onlu
    Muş Alparslan University, Turkey

    Harun ONLU is graduated from the Biology department of YüzüncüYıl University and he completed his master degree in the same university. Later, he was started his PhD in Biology Department of Ankara University in 2011 under the supervision of Prof. Dr. OzlemOsmangaoglu. During the years between 2011-2016, he worked as a research assistant in this department. While he was doing his PhD, he went to Belgium as a researcher in the Universitécatholique de Louvain, Institute of life science Pascal Hols group by getting scholarship fromthe Scientific and Technological Research Council of Turkey (TUBİTAK) between the years of 2015-2016, to carry out some experiments of his PhD thesis. His PhD works are still continuing. He is currently research assistant in theMuşAlparslan University, Department of Molecular Biology and Genetics. His working areas are about probiotic bacteria, bacteriocins, molecular genetics, cloning and knock-out of lactic acid bacteria and lactic acid production.



    Abstract
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    Abstract

    Harun Onlu
    Muş Alparslan University, Turkey

    ABSTRACT: In thisstudy, a promising probiotic bacterium Pediococcuspentosaceus OZF, isolated from human breast milk in our previous studies was encapsulated with calcium alginate (2%) and whey (5%) proteins, followed by lyophilization. Surface morphologies of the prepared microcapsules were analyze dunder scanning electronmicroscope. Alginate capsules containing OZF cells were coated with whey proteins and were found to be stable at 4°C for 6 months without loss of any viability, and encapsulated OZF was found to be viable in simulated gastric and intestinal juice conditions such as pH, bile salts, pepsin andpancreatin. Transit of capsulated and noncapsulated OZF through gastrointestinal system (GIS), the iradhesions as well as their effects on faecal microbiota variations were analyzed on murine model (Balb/C mice) by microbiological counts, qPCR and DGGE, respectively. OZF strain was detected in feacal samples of animal followed by 6 hours after feeding, reached the highest value in 10th hours and started to decrease after and lost completely at 24 hr. At 24 hours following the 15th day of feeding, the number of bacteria adhered to the intestine was determined. At the beginning of thetrial, 8 log cfu was applied per animal and 2 log was found in the group feed with non encapsulated OZF, which means there was 6 log decrease in the adhesion of OZF to GIS at 24 hr. Adhesion properties of encapsulated strain was also found to be the same (2.5log, p>0.05). As far as the feacal microbial composition on the general microbiota is concerned in terms of dominant groups such as Bifidobacteria, Lactobacillus, E.coli and Bacterioides, no significal diffecences were recorded between control and experimental groups.

    Time:

    Title: Cheese matrix effect on proteolysis of P. freudenreichii immunomodulatory proteins

    Gwenael Jan
    INRA-Agrocampus West Rennes, Science and Technology of Milk and Egg, France.

    Biography
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    Biography

    Gwenael Jan
    INRA-Agrocampus West Rennes, Science and Technology of Milk and Egg, France.



    Abstract
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    Abstract

    Gwenael Jan
    INRA-Agrocampus West Rennes, Science and Technology of Milk and Egg, France.

    Propionibacterium freudenreichii is an emergent probiotic, presenting several beneficial effects including anti-inflammatory properties, mediated by surface layer proteins (SLPs) belonged to the S-layer lattice, in particularly the protein SlpB. We hypothesize cheese matrix may be the best delivery vehicle for P. freudenreichii’s anti-inflammatory potential, by enhancing propionibacteria survival to digestives stresses, and by allowing undamaged SLPs to reach the digestive tract. Firstly, we compared the immunomodulatory effects of P. freudenreichii and intact SLPs, to SLPs digested by trypsin, i.e SLPs-peptides on HT29-cells. In contrast to P. freudenreichii and SLPs, SLPs peptides don’t reduce pro-inflammatory cytokines expression during cells co-stimulation with lipopolysaccharide. This result confirmed the importance to protect SLPs from proteolysis to permit them to trigger gut immune system. Secondly, we evaluated propionibacteria resistance and slpB proteolysis during in vitro static digestion, in different delivery vehicles increasingly concentrated in dairy proteins: milk ultrafiltrate, milk and cheese. The experiment clearly showed the protective effect of the Slp proteins by the cheese matrix and better bacterial viability. Then, we carried out in vitroa dynamic digestion using Didgi® system. We selected two matrices only: the milk ultrafiltrate and the cheese. We decided to apply fixed digestion parameters for both matrices: the digestive parameters of cheese. The results show a protective effect of the cheese matrix on the viability of the propionic bacteria and on SLPs against digestive proteolysis. Taken together, those results show that cheese is an adequate delivery vehicle for P. freudenreichii immunomodulatory proteins.

    Time:

    Title: Anti-obesity effects of probiotic Pediococcuspentosaceus OZF on 3T3-L1 Adipocytes

    Harun Onlu
    Muş Alparslan University, Turkey

    Biography
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    Biography

    Harun Onlu
    Muş Alparslan University, Turkey

    Harun ONLU is graduated from the Biology department of YuzuncuYıl University and he completed his master degree in the same university. Later, he was started his PhD in Biology Department of Ankara University in 2011 under the supervision of Prof. Dr. OzlemOsmangaoglu. During the years between 2011-2016, he worked as a research assistant in this department. While he was doing his PhD, he went to Belgium as a researcher in the Universitécatholique de Louvain, Institute of life science Pascal Hols group by getting scholarship fromthe Scientific and Technological Research Council of Turkey (TUBİTAK) between the years of 2015-2016, to carry out some experiments of his PhD thesis. His PhD works are still continuing. He is currently research assistant in theMuşAlparslan University, Department of Molecular Biology and Genetics. His working areas are about probiotic bacteria, bacteriocins, molecular genetics, cloning and knock-out of lactic acid bacteria and lactic acid production.



    Abstract
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    Abstract

    Harun Onlu
    Muş Alparslan University, Turkey

    Since adipocyted if ferentiation and subsequent extend in fat accumulation is closely related to the occurrence of various diseases, inhibiting the proliferation and differentiation of fatcells is thought to be an important strategy in the treatment of obesity.Therefore, it is the aim of this study to investigate the anti-adipogenic effect of probiotic P. pentosaceus OZF isolated from human breast milk on 3T3-L1 (ATCC CL-173™) preadipocytes, as an in vitro model of adipogenesis. 3T3-L1 cells (2x103cell/well) were cultured and allowed to differentiate with different concentrations of lyophilized viable cells as well as cell free supernatant for 10 day sunder 5% CO2 conditions. Following incubation, XTT assay was used to determine the cellular growth and cytotoxicity. The cellular lipid content was also assessed by Oil Red-O staining. Proliferation and differentiation of adipocyte cells were shownto be inhibited by P. pentosaceus OZF as assessed by reduction both in the number of lipid-containing rounded cells and lipid accumulation in fully differentiated 3T3-L1 adipocytes. Our findings suggested that P. pentosaceus OZF might be used as an important strategy for the potential treatment of obesity and further in-vivo studies are needed to elucidate the mode of action.

    Time:

    Title: Using Lactic Acid Bacteria as an Alternative Tool to Improve Honey Bee Health

    Ozlem Osmanagaoglu
    Ankara University, Turkey

    Biography
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    Biography

    Ozlem Osmanagaoglu
    Ankara University, Turkey

    Ozlem Osmanagaoglu graduated from the Biology Department of Middle East Technical University in Turkey. Later, she completed her master and PhD program on genetics of Lactic Acid Bacteria at the Biotechnology Institute of METU. During her doctoral and post doctoral periods, she worked under NATO grant and scholarship in the University of Wyoming, USA. Presently, she is a member of the Biology Department within the Science Faculty of Ankara University in Turkey where she has established Microbial Genetics Laboratory with a young productive research team. Her work has focused on bacteriocins, natural antimicrobial peptides, produced by Lactic Acid Bacteria, their molecular typing and probiotics.



    Abstract
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    Abstract

    Ozlem Osmanagaoglu
    Ankara University, Turkey

    Following China, Turkey is the second country in the world’s honey production with about 7.5 million hives. As of 2016 in Turkey(a geneticcenter of 20% of world bee genotypes, among which Apismelliferassp. are in very important concern), honey average per hive is 14 kg. Although our country has such rich diversity in bee populations and vegetation, it has not reached the desired level in the production of bee products because of bee diseases caused especially by pathogens such as Nosemasp. Rather than the use of traditional methods including the use of antibiotics, alternative options such as the use of Lactic Acid Bacteria (LAB) isolated from bee gastrointestinal system, showing probiotic properties are gaining importance. Therefore, it is the aim of this study to isolate and characterize the beneficial LAB from gut microbiota of honey bees(Apismellifera) collected from different hives in Hatay and Ankara provinces, evaluate their probiotic properties and effecton honey bee health against Nosema infection. So far, in our on going studies, we were able to cultivate and characterize different LAB strains from gut and identified them by 16S rDNA sequencing. We are currently testing the probiotic properties of selected strains andtestingtheireffectson Nosema-infected honey bee samples by counting Nosemaspores in gut.The strains causing reduction in infection will be further used in microbiota analysis by next generation sequencing and in product development. As far as the results obtained so far are concerned, it seems promising to use honey bee gut isolated LAB as an alternative option to treat honey bee diseases.

    Time:

    Title: Characterization of bacteriocin from indigenous probiotic Lactobacillus with emphasis on broad antibacterial spectrum

    Debashis Halder
    University of Gour Banga, India

    Biography
    χ

    Biography

    Debashis Halder
    University of Gour Banga, India

    Debashis Halder is involved in Ph. D Research since last three years, under the supervision of Prof. Shyamapada Mandal, Laboratory of Microbiology and Experimental Medicine, Department of Zoology, University of GourBanga, India.He has worked on three projects and published five research articles in four reputed international journals. He is passionate to pursue a career in Medical Microbiology and truly attracted by Molecular Immunology and Phytomedicine. Hewishes to continue as a scientist with a dynamic team of sincere researchers along with continuous research in his fields and extend his valuable service towards the scientific community with extensive research work.



    Abstract
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    Abstract

    Debashis Halder
    University of Gour Banga, India

    Abstract Background:Bacteriocinsfrom lactic acid bacteria (LAB) have been excellent biotheraputic agents to be used against pathogenic bacteria. However, the antibacterial activities of bacteriocins from indigenous LAB have not been validated scientifically from our part of the globe. The current study determines the antibacterial properties and characterizebacteriocin isolated from local Lactobacillus strains. Methods: Five commercial curd samples were processed microbiologically, using MRS media, in order to isolate LAB. The LAB were identified following conventionaland molecular methods, and the probiotic features were validated. Antagonistic property of the isolated LAB were determined against the indicator pathogenic bacteria. Bacteriocin isolated from such probiotic strains were characterized through enzyme treatment, SDS-PAGE analysis and antibacterial property. Result: The LAB isolates were identified as Lactobacillus Spp. and the isolates showed probiotic features, with tolerance to variable temperature (15°C and 42°C) and acid (pH 2-4), bile salt (0.125%, 0.25% and 0.50 %) and NaCl (2%, 4% and 6.5%). The LAB were γ-hemolytic and did not cause gelatin hydrolysis and mostly were sensitive to the antibiotics tested. Antagonistic activity test revelsa zone diameter of inhibition (ZDI) of 16-46 mm and 7-26 mm in agar-overlay and agar-well techniques, respectively, against the gram positive and gram negative indicator bacterial pathogens. The isolated bacteriocin from probiotic LAB were of 11-14 kDa. The bacteriocin showed antibacterial activity displaying ZDIs 15-30 mm and minimum inhibitory concentration (MICs) 7.12 – 483.12 µg/ml. Conclusion: The lactobacilli were excellent probiotics, and thebacteriocin from the LAB had a broad spectrum of antibacterial activity. Thus, the indigenous Lactobacillus strains from local niches and the bacteriocin isolated from them might be used as biotherapeutic agents against bacterial infection.

    Time:

    Title: Lactobacilli probiotics as a potential control strategy for Avian Intestinal Spirochaetosis.

    Jade Passey
    University of Surrey, UK

    Biography
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    Biography

    Jade Passey
    University of Surrey, UK



    Abstract
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    Abstract

    Jade Passey
    University of Surrey, UK

    Brachyspira species are the causative agents of avian intestinal spirochaetosis (AIS). AIS is a gastrointestinal disease whereby Brachyspira colonise the caeca and colo-rectum of poultry, primarily layer hens, resulting in diarrhoeal disease and consequently a 6-10% reduction in egg production, poor egg quality and faecally stained eggs. It is estimated that AIS costs the UK poultry industry £18 million per annum. Prevalence of Brachyspira in the UK has increased significantly in recent years, with up to 90% of free range hens and 74% of barn hens testing positive for Brachyspira species. Furthermore emerging antimicrobial resistance is a concern. Therefore, a better understanding of the pathobiology and novel measures to mitigate this economically important disease are urgently required. Eight avian Brachyspira isolates from the four clinically relevant species, B. pilosicoli, B. intermedia, B. alvinipulli and B. innocens were selected and subjected to genetic and metabolic analysis using the Biolog system and next-generation whole genome sequencing, in order to further understand the genus. Additionally, to investigate the mechanisms by which Lactobacillus may mitigate AIS, sixteen Lactobacillus isolates were isolated from chicken faeces and characterised using 16S rRNA sequencing, biochemistry and next-generation sequencing. Lactobacillus isolates were screened for in vitro antimicrobial activity against Brachyspira species. These studies indicated that the cell free supernatant (CFS) from all Lactobacillus strains significantly inhibited Brachyspira growth in a pH-dependent manner (p ≤0.01). Furthermore, the CFS from three Lactobacillus strains consistently inhibited the growth of all test Brachyspira strains in a pH-independent manner (p ≤0.01). This suggests that the inhibition of Brachyspira is not solely attributed to acidity. Subsequently, nuclear magnetic resonance spectroscopy (NMR) was used to identify metabolites secreted into the CFS which may have the potential to inhibit Brachyspira. In addition to lactic and acetic acid, other metabolites such as acetoin were identified as having potential antimicrobial properties. Furthermore, these three Lactobacillus isolates were able to physically interact with Brachyspira in order to significantly impair growth and motility (p≤0.05). Collectively, these data suggest that physical interactions between Lactobacillus and Brachyspira are important factors in mitigation of disease and that secreted compounds from Lactobacillus have inhibitory effects against Brachyspira. Further study to explore these mechanisms is required.

    Time:

    Title: Characteristics Of Lactic Acid Bacteria Isolated From Dadih Batu Bajanjang, Lembang Jaya, Solok District, West Sumatra

    Endang Purwati
    University of Andalas, Padang, Indonesia

    Biography
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    Biography

    Endang Purwati
    University of Andalas, Padang, Indonesia



    Abstract
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    Abstract

    Endang Purwati
    University of Andalas, Padang, Indonesia

    Dadih is a fermented product from buffalo milk using bamboo tubes. It is one of the innovative product that provides positive effects for human health with probiotics as requirements of fungsional food. The study aimed to characterize and determine the species of lactic acid bacteria isolated from dadih which has proven produced the best quality of probiotics located in Batu Bajanjang, Lembang Jaya, Solok District, West Sumatra, Indonesia. The research had been performed using the sample from different location (AN, AS, KI). This method identifying by macroscopic, microscopic and molecular using 16S rRNA technique. Furthermore, this research also determine nutritional content such as protein, fat, water content, pH and acidity. The result of nutritional content was not significant different for all sampel. The protein content are AN 5.58%, AS 6.08%, KI 6.68%, fat content are AN 6.4%, AS 7.0%, KI 7.2%, water content are AN 80%, AS 73%, KI 65%, pH are AN 4.14, AS 4.07, KI 4.02 and acidity are AN 1.35%, AS 1.71%, KI 2.12%, respectively. The number of lactic acid bacteria are AN 20.8x107CFU/g, AS 15.3x107CFU/g, KI 3.8x107CFU/g. However, the number of aerobic bacteria are AN 14.8x104CFU/g, AS 46.4x104CFU/g, KI 50x104CFU/g. The results showed the isolate is namely Lactobacillus fermentum strain NCC2970 with morphology bacil, gram-positive with catalase negative. It was potential a commercial starter to produce another fermented food.

    Time:

    Title: Review on the microorganisms isolated from cultured Fish Farm in Abbassa and can be used as a probiotic in cultured Oerochromis niloticus

    Azza M. M. Abd El-Rhman
    Fish Health CLAR, ARC Egypt

    Biography
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    Biography

    Azza M. M. Abd El-Rhman
    Fish Health CLAR, ARC Egypt



    Abstract
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    Abstract

    Azza M. M. Abd El-Rhman
    Fish Health CLAR, ARC Egypt

    The review was conducted with the microorganisms (bacteria, yeast and algae) whish isolated from cultured fish and water ponds in Abbassa Fish Farm and can be used as probiotics for Oeriochromis niloticus by enhancing growth immunity and fish resistant against pathogenic bacteria. Bacillus pumilus, Citrobacter freundii, B. firmus, Micrococcus luteus and pseudomonase species were isolated from O. niloticus and had antibacterial effect against Aeromonans hydrophila in vitro. Lactococcus lactis, Lactobacillus animalis, L. plantarum, L. fermentum, L. raffinolactis and L. acidophilus were isolated from O. niloticus, and common carp intestine and had antibacterial effect against A. cavie and Pseudomonas fluorescence. B. pumilus appeared to be a promising probiotic for controlling A. hydrophila infection. Micrococcus luteus enhanced the growth performance and fish-resistance against A. hydrophila infection. Lactococcus lactis, Lactobacillus animalis enhanced immune response and fish resistance against A. cavie and Ps. fluorescence. Live Saccaromyces castelli and dead Rhodotorula minuta which isolated from cultured fresh water fish in Abbassa Fish Farm had probiotic effect in O. niloticus by enhancing growth performance, feed utilization, immune responses as well as the resistance to A. sobria and P. fluorescence infection. Oscillatoria curviceps and Anabaena wisconsinense (Cyanobacteria) were isolated Abbassa from fish farm water and its extracts had antibacterial activity against A. hydrophilia, Ps. fluorescence, Ps. anguilliseptica, B. firmus, Aspergillus niger and Saprolegnia parasitica in vitro. Anabaena wisconsinense hall cell supplemented diet (1%) had immunostimulant effect in O. niloticus and enhanced fish resistance against A. veronii, A. sobria and Actinobacter anitratus.

    Time:

    Title: Screening of purine nucleosides degrading probiotic lactic acid bacteria as a novel approach in management of hyperuricemia

    Neha Pandey
    National Dairy Research Institute, India

    Biography
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    Biography

    Neha Pandey
    National Dairy Research Institute, India

    Dr. Neha Pandey is working as DST Women Scientist-A in Dairy Microbiology (DM) Division in National Dairy Research Institute (NDRI), Karnal, Haryana, India since 24th June 2016 under the mentorship of Dr.ShilpaVij (Principal Scientist, NDRI, Karnal). She has completed her Ph.D in Dairy Microbiology from NDRI Karnal in 2013 and has eight publications in journals of national and international repute. For carrying out present study the authors highly acknowledge the funds received from Department of Science and Technology (DST), India.



    Abstract
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    Abstract

    Neha Pandey
    National Dairy Research Institute, India

    Out of 20 different curd and fecal samples, 76 Gram positive and catalase negative rods were isolated and screened for purine nucleoside degradation ability. A HPLC device equipped with a Photo diode array (PDA) detector was used to evaluate the ability of these isolates to degrade inosine and guanosine, the two key intermediates in purine metabolism by incubating these isolates with inosine-guanosine solution (1.25mM) for 2 h at 37oC. Contents of remaining inosine and guanosine were identified at 254 nm by retention time of 7.48 and 6.46 min, respectively, and quantified by interpolation of calibration curves. To evaluate the degradation of purine compounds by cell-free extracts of LAB, the cells were sonicated for 30’X3 (pulse rate- 5sec on/ 5 sec off) and activity evaluated through HPLC. Eleven isolates viz. D3A, D1AB, D1F, D3E, D6F, D2A, D5F, D3D, D3B, D1AC, D1AA were screened as positive for purine nucleoside degradation ability. These organisms were then evaluated for their potential probiotic attributes. Out of these 11 isolates only six isolates viz. D3A, D1AB, D2A, D6F, D5F and D1AA were found to be most acid tolerant and selected for further in vitro evaluation. When tested for bile tolerance only three isolates viz. D6f, D5F, D1AA and D3A were found to resist 2% bile and showed highest viability among the tested six strains. Further evaluation of the isolate for antimicrobial activity showed that the all the screened four isolates showed a good antimicrobial activity against Gram positive bacteria viz. Enterococcus faecalis, Bacillus subtilis, Staphylococcus aureus, Microccusluteus and other Lactobacillus species. Our work shows that our isolates apart from purine degradation are good probiotics as proved by their acid and bile tolerance. Also, the antimicrobial activity showed by the isolate can give an added advantage to the isolate to compete and survive in the GI tract.

    Time:

    Title: Wine Lees Based Functional Beverage as a New Source of Probiotics with Possible In Situ Oenological Valorising

    Teodora Emilia Coldea
    University of Agricultural Sciences and Veterinary Medicine, Romania

    Biography
    χ

    Biography

    Teodora Emilia Coldea
    University of Agricultural Sciences and Veterinary Medicine, Romania



    Abstract
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    Abstract

    Teodora Emilia Coldea
    University of Agricultural Sciences and Veterinary Medicine, Romania

    Food industry by-products represent important alternative sources of probiotics. Wine lees containing strains of Saccharomyces cerevisiae, are commonly used as probiotic supplements in animal feed and recently in human nutrition. They contain many proteins, carbohydrates and vitamin B complex. One of the most important probiotic properties of yeast is their capability to control the pathogenic microorganisms, by creating equilibrium in intestinal microflora. So, it is understandable the last decade trend to valorise this winery by-product into a functional value-added product. This study assessed the adaptation of wine yeast strains from wine lees to gastrointestinal conditions by using an emerging technique - encapsulation. The most frequent way wine lees are used is in solid functional products. We aimed both the valorising the wine lees into a novel functional beverage, and testing its probiotic effect into simulated gastrointestinal conditions. Three different testing variants were performed: encapsulation by spray-drying, lyophilized and raw material collected from the bottom of the tank, when fermentation process ended. Spray-drying and lyophilisation variants proved to be suitable for the probiotic protection, thus these technologies are widening the utilisation of winery valuable by-products in food industry.

    Time:

    Title: Probiotic lectin systems potential: the glycoconjugate binding strategies

    Vladimir Lakhtin
    G.N.Gabrichevsky Research Institute for Epidemiology and Microbiology, Russia

    Biography
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    Biography

    Vladimir Lakhtin
    G.N.Gabrichevsky Research Institute for Epidemiology and Microbiology, Russia

    Vladimir Lakhtin has his expertise in study of glycoproteins, lectins and their complexes, multistrain probiotics, antimicrobials, and innate immunity (complement system, etc.) upon infectious and non-infectious diseases. His functional evaluation models based on glycoconjugate recognition and responses of human biotope microbiocenoses and innate protective systems creates new perspective possibilities in diagnostics and prognostics in the field of medical biotechnology and clinical microbiology.



    Abstract
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    Abstract

    Vladimir Lakhtin
    G.N.Gabrichevsky Research Institute for Epidemiology and Microbiology, Russia

    Innate protection involving lectin systems is of increasing attention of investigators. The human protection using glycoconjugates (GC) recognizing systems is important immune ingredient against infectious and autoimmune diseases. Probiotic lectin systems (PLS) possess multiple directions of actions useful for human. The aim was to propose the GC-strategies for evaluation the strain potential to select strain ingredients and construct probiotics. Materials and methods: Probiotic bacteria were from collection of G.N.Gabrichevsky Research Institute for Epidemiology and Microbiology. PLS were identified and isolated using isoelectrofocusing in polyacrylamide gel. They were identified on blots using GC-biotin (www.lectinity.com) and streptavidin-peroxidase in the presence chemiluminescent substrate in a real time in BioChemi System (UVP). Proteins were localized with SYPRO protein blot stain (Bio-Rad). Results and discussion: Different GC-binding PLS (> 27 kD, pI 4-8) were identified and isolated. PLS were localised as mosaics within protein massifs. The use of GC (www.lectinity.com) creates basis for constructing metabiotic and metabolomebiotic therapeutics of system action. The following activities of strain metabolites can be predicted: antipathogenic activities (the use of GC imitating microbial cell surface and wall structures: peptidoglycans, sialylated glycans, glycoantigens, others); prebiotic/ synbiotic actions (GC imitating prebiotic structures: derivatives of L-fucans; modified D-galactoside oligomers, etc.); increasing human protection (GC regulating peritoneal macrophages, complement system lectin components: (phospho)mannans and other polysaccharides, glycoantigens); on-duty support against cancerogenesis (normal cell surface décor panels of GC delivered); on-duty support of immunomodulation (panels of GC as antigens: peptidoglycans, others). PLS effectively imitated antimicrobial activities of bifidobacterial and lactobacillar cell probiotics. Conclusions: Results indicate the prospects of PLS as the new important functional metabolite ingredients of probiotics to use for prophylaxis and therapy of infectious and non-infectious diseases. The choice of fine specific (PLS—GC)-type interactions needed can be extended using a broad panel of GC.

    Time:

    Title: Lactobacillus plantarum inhibits mouse colonic inflammatory response by activating NLRP6

    Chunfeng Wang
    Jilin Agricultural University, China

    Biography
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    Biography

    Chunfeng Wang
    Jilin Agricultural University, China

    Professor Wang graduated from Jilin Agricultural University with master's degree in Preventive Veterinary Medicine. Then she attended China Agricultural University to earn the Ph.D. degree. After the doctoral career in Beijing, she was admitted to Harvard University to pursue her post-doctoral research. During the last two decades, she has devoted herself in the research work for the development of novel animal micro ecological agents, construction of a mucosal delivery platform to express heterologous proteins utilizing engineering lactic acid bacteria which have been regarded as safe microorganisms, exploration of molecular immune mechanisms for the new functional lactic acid bacteria, preparations of environmentally friendly animal microecological formulations. All the initial innovative academic achievements have been published in the journals, such as Cell Transplantation, Virology, and Scientific Reports.



    Abstract
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    Abstract

    Chunfeng Wang
    Jilin Agricultural University, China

    Many experimental with animal models, clinical trials, and cell culture studies they have been confirmed that Lactobacillus plantarum has the ability to inhibit the inflammatory response, however, its mechanism to inhibit the inflammatory response is unclear. In this study we used LPS to treat BMDM and then added Lactobacillus plantarum in it. The results suggest that Lactobacillus plantarum could promote the expression of NLRP6 in BMDM cells and form inflammatory complexes with Caspase-1 and ASC, which inhibit the secretion of extracellular interleukin (IL)-1β, and promote the secretion of extracellular IL-18. We also used siRNA to interfere with the expression of NLRP6 in BMDM, the result showed that promote the secretion of IL-1β and inhibit the secretion of IL-18. Importantly, pre-inoculation with Lactobacillus plantarum can effectively attenuate DSS-induced colitis in mice through the result of colonic scoring, colon pathological pathological changes and weight loss indicators. In conclusion, Lactobacillus plantarum inhibits the inflammatory response by activating the Nod-like receptor (NLR) family member, NLRP6.This study have laid the foundation for the application of lactic acid bacteria in the treatment of colitis.

    Time:

    Title: Identification of four probiotic strains isolated aboriginal and their antimicrobial power potency against Staphylococcus aureus

    Mohamed Nadjib Boulmaali
    University of BLIDA 1, Algeria

    Biography
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    Biography

    Mohamed Nadjib Boulmaali
    University of BLIDA 1, Algeria



    Abstract
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    Abstract

    Mohamed Nadjib Boulmaali
    University of BLIDA 1, Algeria

    In most countries, the contamination of food represents a major problem for the consumer; the feat of bacterial interactions is a new way to fight against the undesirable germs The objective of this study is therefore the insulation of probiotic bacteria aboriginal from local products (cow's milk, goat's milk and dried red-salted- meat “kedid: Traditional Algerian product”) suspected of antimicrobial activity in order to identify and prove its antimicrobial abilities against Staphylococcus aureus taken from a urinary tract infection. For our research we have selected four isolates V6 "cow's milk", C2 "goat's milk", K1 and K2 "kedid" with the following characteristics: Gram-positive lactobacilli, catalase negative, stationary and grow in anaerobic. The biochemical results confirm that are: lactobacillus plantarum, lactobacillus fermentum, lactobacillus sakei and lactobacillus animalis in order [1]. The antimicrobial potency of these strains against Staphylococcus aureus was significant for three isolates: V6, C2 and K1 with diameters of 6 to 9 mm (V6), 8 to 14 mm (C2) and 22 to 29 Mm (K1) with this power the three isolates can play an important role to minimize the contamination of food[2].

    Time:

    Title: A Lipid Extract Increases the Viability of Encapsulated Lactobacillus caseiDuring Heat Treatment and Simulated Gastrointestinal Passage

    Oana Lelia Pop
    University of Agricultural Science and Veterinary Medicine

    Biography
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    Biography

    Oana Lelia Pop
    University of Agricultural Science and Veterinary Medicine

    Pop Oana Lelia (33-year-old) is a Teaching Assistant at the University of Agricultural Science and Veterinary Medicine from Cluj and works at the Faculty of Food Science and Technology as academic since 2016 and researcher since 2012. Her Ph.D. theses were developed on probiotics encapsulation, during that time she worked for 6 months in Germany in an enterprise on the subject. Her research work is related with fermentation, nanoparticles, plant extracts besides the main subject - pro and prebiotics encapsulation.



    Abstract
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    Abstract

    Oana Lelia Pop
    University of Agricultural Science and Veterinary Medicine

    Probiotics are the bacteria that can provide health benefits to the consumers and they are suitable to be added to a variety of foods. In this research, viability of immobilized Lactobacillus casei in alginate with or without sea buckthorn lipid extract were studied during heath treatment and with an in vitro gastrointestinal model. The characterisation of the lipid extract was also done using the UV-Vis spectrometry (UV-Vis), high-performance liquid chromatography photodiode array detection method (HPLC-PDA), gas chromatography coupled with mass spectrometry (GS-MS) and Cryo scanning electron microscopy (Cryo-SEM). During the heat treatment, the entrapped probiotic cells proved higher viability even at temperatures above 50 °C (> 6 log CFU/g). The rich in monounsaturated fatty acids sea buckthorn fraction improve the in vitro digestion passage regarding the probiotic viability. The survival of the probiotic cells was 15 % higher after 2 h in the acidic medium of the simulated gastric fluid in the sample where L. casei was encapsulated with the sea buckthorn extract compared with the samples where no extract was added. Thus, this study may be effective for the future development of probiotic-supplemented foods as foods with health welfare for the consumers.

    Time:

    Title: Invitro and Invivo Safety analysis of Enterococcus facieum 2C isolated from Breast Milk

    Soodabeh Khalkhali
    Razi vaccine and Serum research institute, Iran

    Biography
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    Biography

    Soodabeh Khalkhali
    Razi vaccine and Serum research institute, Iran

    Ms Soodabeh Khalkhali, born on January 2nd, 1981 in Tehran Iran, is a Ph.D candidate (Microbiology) at Islamic Azad University Shiraz Iran. Her masters and PhD thesis has been on Probiotic bacteria and has published couple of papers on the importance of enterococcus species and significance of candidate probionts in mothers’ milk. Ms Khalkhali is also a part time teacher at a university in Tehran.



    Abstract
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    Abstract

    Soodabeh Khalkhali
    Razi vaccine and Serum research institute, Iran

    analysis of the probiotic bacteria is an obligatory characteristic evaluated prior to introducing them to food or pharmacological industry. This study was designed to evaluate in vitro and in vivo safety of Enterococcus faecium, a probiotic candidate isolated from human breast milk E.faecium isolated from breast milk was studied for its hemolytic activities, phenotypic antibiotics resistance profile and antibacterial activities in in vitro condition. Duringinvivo investigations, the oral toxicity of the mentioned probiotic strain was evaluated in Wistar Male rats. The animals were fed daily with dose of 1 x 1011 CFU/kg of body weight, respectively, for 21 consecutive days and their hematological, biochemical parameters, organ weight, body weight and common health features were recorded. The results revealed that E.faecium 2C was non-hemolytic, sensitive to majority of tested antibiotics and was able to inhibit the growth of several pathogenic bacteria. During invivo investigations, the Wistar male rats fed orally fed rats survived during the test period and showed normal growth and development. There were no adverse effects on the general condition, behavior, growth, feed and water consumption, hematology, clinical chemistry values, organ weights and histopathologic analysis of the rats. Results of this study demonstrate that consumption of strain E. faecium2C, even in large quantities, is not associated with any obvious signs of toxicity in Wistar rats. All physiological and biological health parameters including body and organ weights, biochemical blood and serum analysis revealed the safety of E.facieum isolate in study. None of the vital organs showed the sign of bacteremia or infectivity in the tested rat models. E.faecium strain isolated from human breast milk is a safe probiotic with several beneficial properties; hence, it can be introduced for use as supplement for man and animals.

    Time:

    Title: Inhibition of Salmonellosis with produced conjugated linoleic acid by linoleate isomerase of rumen fungus

    Rabar M. Abdulrahman
    Kahramanmaras Sutucu Imam University, Turkey

    Biography
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    Biography

    Rabar M. Abdulrahman
    Kahramanmaras Sutucu Imam University, Turkey

    Rabar M. Abdulrahman is a PhD Student completed his Masters Medical Microbiology from the University of Ulster. He then worked at the Medical Microbiology Department, Koya University, served as Assistant Lecturer at the University in IRAQ. He has been working on antibacterial resistance pattern within Salmonella sp. Currently he is working on conjugated linoleic acid produced by rumen fungi and their effect on Salmonella sp.



    Abstract
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    Abstract

    Rabar M. Abdulrahman
    Kahramanmaras Sutucu Imam University, Turkey

    Department ofapplied Science,Kahramanmaras Sutucu Imam University, Kahramanmaras, TURKEY, 46000. During the last two decades, the epidemiology of foodborne pathogens has changed rapidly along with the alterations in the social atmosphere and the ability of pathogens to adapt to new niches. Food is a source of transmitting diseases through which more than 200 diseases are transmitted. Salmonella is the most commonly suggested cause of foodborne disease which constitutes a major public health problem in many countries. In this study, we have isolated anaerobic fungi (Orpinomyces sp. and Neocallimastix sp.) from rumens feces. Then by growing them within medium containing only Linoleic Acid as a source of energy, we have identified those that are capable of converting Linoleic acid into Conjugated linoleic acids. The produces CLA (conjugated linoleic acid) has been used as antibacterial to show their effects of Salmonellosis. The result of this study, showed that the produced CLA has antibacterial effect on all four strains of Salmonella sp. (Salmonella enterica, Salmonella Typhi ATCC 14081, Salmonella sp.1 and Salmonlla sp.2) used within this study.

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